聚酰胺纯化膜苞鸢尾中总黄酮的工艺研究

Purification of Total Flavonoids From Iris scariosa by Polyamide Resin

目的研究聚酰胺柱色谱纯化膜苞鸢尾中总黄酮的工艺条件。斱法以膜苞鸢尾中总黄酮的解析率与纯度为指标,通过考察静态、动态吸附试验,优化聚酰胺纯化膜苞鸢尾中总黄酮的工艺条件。结果聚酰胺对总黄酮有良好的纯化效果,其优化工艺为:最佳上柱样品溶液质量浓度2.0mg·mL-1,pH=3,吸附流速1 BV·h-1,吸附1.5 h后,70%乙醇洗脱液以1 BV·h-1速度洗脱。总黄酮动态解析率为92.34%,纯度45.59%。结论聚酰胺对膜苞鸢尾中总黄酮纯化性能较高,纯度提高了2.9倍。

Objective:To optimize the purification method of total flavonoids from Iris scariosa by polyamide resin.Methods:Using the desorption ratio and purity of total flavonoids from Iris scariosa as the indices,the purification of total flavonoids by polyamide resin was optimized with static and dynamic adsorption tests.Result:The optimal adsorption conditions were as follow:the concentration of sample solution was 2.0 mg·mL-1,pH was 3 and the flow velocity of adsorption was 1 BV·h-1,the sample solution was rested for 1.5 h in the chromatographic column of polyamide resin.70%ethanol was used to elute the total flavonoids at the flow rate of 1 BV·h-1.The dynamic desorption ratio was 92.34%and the purity of total flavonoids was 45.59%.Conclusion:The total flavonoids in Iris scariosa was effectively purified by polyamide resin under the optimal conditions with 2.9 times increase.