摘要
为了研究全蝎白术白头翁组方(SAPZF)发酵前后最佳萃取部位,采用球孢白僵菌双向固体发酵技术制备全蝎白术白头翁组方发酵物(SAPZFFW),分别制取组方发酵前后的质量分数75%醇提液。采用系统萃取法,制得SAPZFFW、SAPZF的石油醚部、乙酸乙酯部、正丁醇部、水部干浸膏。通过MTT法检测SAPZFFW、SAPZF不同极性部位对乳腺癌细胞株MCF-7的抑制作用。结果表明:SAPZF有效部位的抗肿瘤能力由大到小依次为,石油醚部、正丁醇部、乙酸乙酯部;SAPZFFW有效部位抗肿瘤能力由大到小依次为,正丁醇部、石油醚部、乙酸乙酯部;SAPZFFW对乳腺癌细胞株MCF-7的抑制作用优于SAPZF,且正丁醇部效果较好。
In order tostudy the optimum extraction site of Scorpio,Atractylodes Macrocephalae Rhizoma and Radix Pulsatillae Prescription(SAPZF)before and after fermentation,SAPZFFW(fermentation product of SAPZF)was prepared by the two-way solid fermentation of Beauveria bassiana.The 75%alcohol extract of the SAPZFFW and SAPZF were subsequently obtained.Their dry extracts of petroleum ether part,ethyl acetate part,n-butanol part and water part were prepared by system extraction,respectively.Their inhibitions on breast cancer cell line MCF-7 were assayed by MTT for different polar sites.The anti-tumor ability of SAPZF effective site in order from large to small was determined as follows:petroleum ether part>n-butanol part>ethyl acetate part.That of SAPZFFW was determined as follows:n-butanol part>petroleum ether part>ethyl acetate part.The inhibitory effect of SAPZFFW on breast cancer cell line MCF-7 was better than that of SAPZF,and the effect of n-butylalcohol part was better.
作者
陶然
王娅玲
李佳怿
刘富菁
陈美琦
王集会
张珊珊
TAO Ran;WANG Ya-ling;LI Jia-yi;LIU Fu-jing;CHEN Mei-qi;WANG Ji-hui;ZHANG Shan-shan(School of Medicine,Shandong University of Traditional Chinese Medicine,Jinan Shandong 250355,China;College of traditional Chinese Medicine,Shandong University of traditional Chinese Medicine,Jinan Shandong 250355,China;Experimental Center,Shandong University of Traditional Chinese Medicine,Jinan Shandong 250355,China)
出处
《当代化工》
CAS
2020年第6期1154-1157,共4页
Contemporary Chemical Industry
基金
山东中医药大学优秀青年基金(项目编号:2018zk34)
山东中医药大学大学生研究训练(SRT)计划项目(项目编号2019042)
关键词
全蝎白术白头翁
系统萃取法
极性部位
发酵
乳腺癌细胞株MCF-7
Scorpio
Atractylodes macrocephalae rhizoma and radix pulsatillae
System extraction
Polar region
Fermentation
breast cancer cell line MCF-7