不同剂量白藜芦醇对糖尿病性白内障大鼠晶状体抗氧化酶活力的影响

Effect of Resveratrol in Different Doses on the Activity of Lens Antioxidant Enzymes in Diabetic Cataract Rats

目的:探究不同剂量白藜芦醇对糖尿病性白内障大鼠晶状体抗氧化酶活力的影响。方法:75只5周龄健康SPF级雄性SD大鼠按照随机数字表法分为正常对照组、模型组,白藜芦醇低剂量组,白藜芦醇中剂量组和白藜芦醇高剂量组,每组各15只。五组大鼠均给予常规适应性喂养,模型组和白藜芦醇低、中、高剂量组大鼠采用链脲佐菌素(STZ)以60 mg/kg的给药剂量制作糖尿病大鼠模型,成模后白藜芦醇低剂量组按20 mg/kg、白藜芦醇中剂量组按50 mg/kg、白藜芦醇高剂量组按100 mg/kg的给药剂量每日给予白藜芦醇灌胃。观察12周后5组大鼠晶状体的混浊程度,检测血糖、体重后处死大鼠,检测晶状体内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)以及过氧化氢酶(CAT)的活性改变。结果:随着白藜芦醇剂量的升高,白藜芦醇低、中、高剂量组大鼠的血糖逐渐降低而体重逐渐升高,且组间比较均具有统计学差异(P<0.05)。三组不同剂量白藜芦醇干预组大鼠晶状体的浑浊程度均低于模型组,且白藜芦醇高剂量组大鼠晶状体的浑浊程度最低,差异均具有统计学意义(P<0.05)。白藜芦醇低、中、高剂量组大鼠SOD、GSH-PX和CAT酶活力与模型组大鼠相比均明显升高,而与正常对照组相比均明显降低(均P<0.05)。随着白藜芦醇剂量的升高,白藜芦醇低、中、高剂量组大鼠SOD、GSH-PX和CAT酶活力逐渐升高,且组间比较均具有统计学差异(P<0.05)。结论:高剂量白藜芦醇可更为明显地降低血糖浓度,提高晶状体SOD、GSH-Px及CAT酶活力,改善糖尿病性白内障晶状体的浑浊程度。

Objective:To investigate the effect of resveratrol in different doseson the activity of lens antioxidant enzymes in diabetic cataract rats.Methods:75 cases of 5-week-old healthy SPF male SD rats were randomly divided into normal control group,model group,low dose resveratrol group,middle dose resveratrol group and high dose resveratrol group according to the random number table,each group had 15 cases.Five groups of rats were given conventional adaptive,and the model group and low,middle and high dose resveratrol group were fed with streptozotocin(STZ)at a dose of 60 mg/kg to make diabetic rats model.After the model was established,the rats in the low dose resveratrol group were fed with 20 mg/kg,middle dose resveratrol group 50 mg/kg,and high dose resveratrol group were fed with 100 mg/kg resveratrol daily.After 12 weeks,the degree of lens opacification was observed in 5 groups,and the rats were killed after blood glucose and body weight were measured,also the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)were detected.Results:With the increase of resveratrol dose,the blood sugar of rats in the low,middle and high dose resveratrol groups decreased gradually and their weight increased gradually,and there was statistical difference between the groups(P<0.05).The degree of lens opacification in the three groups was lower than that in the model group,and the high dose resveratrol group had the lowest degree of lens turbidity,the difference was statistically significan(P<0.05).The activities of SOD,GSH-PX and CAT in low,middle and high dose resveratrol groups were significantly higher than those in the model group,but significantly lower than those in the normal control group(P<0.05).With the increase of resveratrol dose,the activities of SOD,GSH-PX and CAT in low,middle and high dose resveratrol groups increased gradually,and there was statistical difference between the groups(P<0.05).Conclusion:High dose resveratrol can significantly reduce the blood glucose concentration,increase the activity of SOD,GSH-PX and CAT in lens,and improve the degree of lens opacification in diabetic cataract.