磁压榨技术建立大鼠胃肠吻合模型的实验研究

An Experimental Study on the Establishment of a Rat Gastrointestinal Anastomotic Model by Magnetic Compression Technique

目的:探讨采用磁压榨技术建立大鼠胃肠吻合模型的可行性。方法:设计加工适用于大鼠胃肠吻合的子、母磁体。将10只SD大鼠采用磁压榨技术进行胃肠吻合,子、母磁体分别经口置入大鼠胃和空肠内,子母磁体相吸压榨胃壁和肠壁,磁体间受压组织缺血坏死后连同磁体从吻合口脱落入肠道,胃肠吻合即建立,磁体最终经消化道自行排出体外,术后2周处死动物,获取吻合口标本,检测吻合口爆破压、肉眼和光镜下观察吻合口愈合情况。结果:10只SD大鼠中,1只因麻醉意外死亡,其余9只大鼠均顺利完成手术操作并存活至术后2周;手术平均操作时间(15.89±3.25)min,磁体排出体外时间(8.56±1.26)天(范围7-11)天;吻合口爆破压均大于200 mm Hg,吻合口组织HE染色和Masson染色可见粘膜层连续性建立,愈合良好。结论:磁压榨技术可用于大鼠胃肠吻合模型制备,具有操作简单、成功率高的优点。

Objective:To explore the feasibility of magnetic compression technique(MCT)in establishing a gastrointestinal anastomosis model in rats.Methods:Magnetic rings(parent and daughter)suitable for gastrointestinal anastomosis in rats were designed and processed.Ten SD rats underwent gastrointestinal anastomosis using magnetic compression technique.The parent and daughter magnetic rings were inserted into the stomach and jejunum respectively through the mouth.The parent and daughter magnetic rings were attracted to compress the stomach and intestinal wall.The magnets with ischemia and necrosis of the compressed tissue between them dropped from the anastomotic stoma to the intestinal tract.Then the gastrointestinal anastomosis was established.The magnets were eventually expelled through the digestive tract.Rats were killed two weeks after the operation.The blasting pressure was evaluated.The healing of anastomotic stoma was observed by naked eye and light microscope.Results:Except one SD rat died accidentally due to anesthesia,the other nine SD rats successfully completed the operation and survived two weeks after operation.The average operation time was(15.89±3.25)min,and the expulsion time of magnetic rings was(8.56±1.26)days(range 7-11 days).The bursting pressure of anastomotic stoma was more than 200 mm Hg.The continuity of the mucosal layer was evaluated by HE staining and Masson staining,and the healing was good.Conclusion:The magnetic compression technique is a simple method of anastomosis that is feasible,fast and has high success rate for gastrointestinal anastomosis model in rats.