超高效液相色谱-四级杆飞行时间质谱法测定运动型功能饮料中的多种氨基酸

Determination of Multiple Free Amino Acids in Sports Functional Beverages by Ultra-High Performance Liquid Chromatography-Quadrupole Time of Flight

目的:基于超高效液相色谱-四级杆飞行时间质谱法,建立一种快速、有效的测定运动型功能饮料中多种游离氨基酸的方法。方法:样品经水稀释后定容,在0.1%甲酸乙腈和0.1%甲酸甲酸铵流动相梯度洗脱条件下,经HILIC-Z色谱柱分离,利用质谱库对样品进行定性分析,采用外标法进行定量分析。结果:15种氨基酸在0.5~4.0μg/m L间均具有良好的线性关系,相关系数R2在0.9900~0.9996之间,检出限可达1~100 ng/mL,加标回收率为93.1%~107.7%,相对标准偏差在1.7%~5.5%之间。结论:该方法具有便捷、准确、灵敏、有效等优点,可用于运动型功能饮料中多种氨基酸含量的实际检测。

Objective:To establish a rapid and effective method for the determination of multiple free amino acids in sports functional beverages based on ultra-high performance liquid chromatography-quadrupole time of flight(HPLC-QTOF).Method:The sample was diluted with water and fixed to volume,under gradient elution conditions of 0.1%formic acid acetonitrile and 0.1%ammonium formate mobile phase,it was separated by HILIC-Z chromatography column,the sample was qualitatively analyzed using a mass spectrometry library,and quantitatively analyzed using external standard method.Result:All fifteen amino acids have a good linear relationship between 0.5μg/mL and 4.0μg/mL,with a correlation coefficient R 2 between 0.9900 and 0.9996,the detection limits can reach 1 ng/mL to 100 ng/mL,the recovery rates ranges from 93.1%to 107.7%,and the relative standard deviations ranged from 1.7%to 5.5%.Conclusion:This method has the advantages of convenience,accuracy,sensitivity and effectiveness,and can be used for the actual detection of various amino acid contents in sports functional beverages.