朝鲜淫羊藿多糖复合酶提工艺优化及抗氧化活性研究

Optimization of Compound Enzyme Extraction Process and Antioxidant Activity of Epimedium koreanum Nakai Polysaccharides

目的:优化复合酶法提取淫羊藿多糖的工艺,同时评价淫羊藿多糖的抗氧化活性。方法:以朝鲜淫羊藿为原料,多糖提取率为指标,利用纤维素酶、果胶酶、木瓜蛋白酶协同作用提取淫羊藿多糖,基于正交试验和响应面试验优化复合酶提取工艺条件,利用DPPH法、ABTS法评价淫羊藿多糖的体外抗氧化活性。结果:朝鲜淫羊藿多糖最佳酶提取工艺为酶添加量(果胶酶8000U/g、纤维素酶17600 U/g、木瓜蛋白酶900 U/g)、提取时间2 h、p H6.0、液料比53 mL/g、提取温度50℃,在此条件下,淫羊藿多糖的得率为(16.95±0.15)%,比水提法高3.86%。且酶提多糖具有更强的体外抗氧化活性。结论:复合酶提的优化工艺条件温和、操作简单,多糖得率和抗氧化活性均较传统水提取法更高。

Objective:To optimize the process of extracting polysaccharides from Epimedium by composite enzyme method and evaluate the antioxidant activity of Epimedium polysaccharides.Methods:Epimedium koreanum Nakai was used as the raw material,and the polysaccharide extraction rate was used as the index.The polysaccharide of Epimedium was extracted by the synergistic effect of cellulase,pectinase,and papain.The extraction conditions of complex enzyme were optimized based on orthogonal test and response surface test.The antioxidant activity of Epimedium polysaccharide in vitro was evaluated by DPPH method and ABTS method.Results:The optimal enzyme extraction process was as follows:enzyme addition(pectinase 8000 U/g,cellulase 17600 U/g,papain 900 U/g),extraction time 2 h,pH 6.0,liquid to material ratio 53 mL/g,extraction temperature 50℃.Under these conditions,the yield of Epimedium polysaccharide was(16.95±0.15)%,3.86%higher than that of water extraction.Moreover,enzyme extracted polysaccharides have stronger in vitro antioxidant activity.Conclusion:The optimized process conditions of composite enzyme extraction are mild,the operation is simple,and the polysaccharide yield and antioxidant activity are higher than traditional water extraction methods.