以氨基酸为基础的不同食品组方对小鼠抗疲劳能力的影响

Effects of different food groups based on amino acids on anti-fatigue ability of mice

目的:设计以氨基酸为主的食品添加剂组方探讨各组方对小鼠抗疲劳能力的影响。方法:选取健康SPF级昆明小鼠90只,体质量(18±2)g,雌雄各半,将小鼠分为9组,一组10只,每只鼠单笼饲养。选取谷氨酰胺、γ-氨基丁酸、天冬氨酸、左旋肉碱+磷脂酰胆碱、VB3+VB6+锌+硒5个因素,按照低剂量、高剂量2水平处理,加入饲料中进行正交试验。同时设置空白对照组,培养1个月。测定小鼠负重游泳的时间、疲劳小鼠血糖值、血乳酸、谷胱甘肽过氧化物酶以及乙酰胆碱酯酶活性。结果:正交模型抗疲劳指标差异极显著(P<0.01),优化组合为谷氨酰胺800 mg/kg·d、γ-氨基丁酸200 mg/kg·d、天冬氨酸400 mg/kg·d、左旋肉碱800 mg/kg·d、磷脂酰胆碱400 mg/kg·d、VB320 mg/kg·d、VB62 mg/kg·d、锌200 mg/kg·d、硒200 mg/kg·d。此组合的血糖值、血乳酸及乙酰胆碱酯酶指标差异极显著(P<0.01),谷胱甘肽过氧化物酶活性差异显著(P<0.05)。结论:研究得到了抗疲劳功效的食品组方,且确定了其具有抗疲劳作用,可为开发具有抗疲劳功能的保健食品提供依据。

Objective:Designing amino acid-based food additives to explore the effects of each group on the anti-fatigue ability of mice.Method:Ninety healthy SPF Kunming mice were selected,weighing(18±2)g,male and female,and the mice were divided into 9 groups,a group of 10,each of which was kept in a single cage.Select glutamine,γ-aminobutyric acid,aspartic acid,L-carnitine+phosphatidylcholine,VB3+VB6+Zinc+Selenium,five factors,according to low-dose,high-dose two levels,added to the feed for orthogonal test.A blank control group was set at the same time and cultured for one month.The time of weight-bearing swimming in mice,BG level of fatigued mice,lactic acid,GSH-Px and Ach E activity in serum were measured.Result:The anti-fatigue index of the orthogonal model was significantly different(P<0.01),Optimized combination of glutamine 800 mg/kg·d,γ-aminobutyric acid 200 mg/kg·d,aspartic acid 400 mg/kg·d,L-carnitine 800 mg/kg·d,phosphatidylcholine 400 mg/kg·d,VB320 mg/kg·d,VB62 mg/kg·d,Zinc 200 mg/kg·d,Selenium 200 mg/kg·d.The BG level,lactic acid and Ach E index of this combination were extremely significant(P<0.01),GSH-Px activity was significantly different(P<0.05).Conclusion:The food composition with anti-fatigue effect was obtained,and it was determined that it has anti-fatigue effect,which could provide a basis for developing health food with anti-fatigue function.