鹰嘴豆总皂苷制备及体外降血糖与抗氧化活性评价

Preparation of total saponins from chickpea and evaluation of hypoglycemic and antioxidant activities in vitro

优化鹰嘴豆总皂苷的提取及纯化工艺,评价其体外降血糖及抗氧化活性。采用响应面法优化超声辅助提取鹰嘴豆总皂苷,并利用大孔树脂对其进行纯化。通过鹰嘴豆总皂苷对α-葡萄糖苷酶抑制作用来评价其体外降血糖活性;以总抗氧化能力、对DPPH·及·OH自由基的清除能力及还原能力评价其体外抗氧化活性。最佳提取工艺为:乙醇浓度68.13%、超声功率106.96 W、超声温度49.86℃、超声时间45.26 min,此条件下提取率为0.4824%。D-101大孔树脂纯化后,鹰嘴豆总皂苷纯度由16.42%提升至27.81%。纯化后的鹰嘴豆总皂苷对α-葡萄糖苷酶抑制作用(IC50=0.2053mg/mL)、总抗氧化能力(TEAC=8.408 mmol/g)、DPPH·清除能力(IC50=0.0667 mg/mL)、·OH清除能力(IC50=1.836 mg/mL)及还原能力均呈现一定的剂量-效应关系,且都强于未纯化的鹰嘴豆总皂苷。通过优化鹰嘴豆总皂苷提取及纯化工艺,其提取率和纯度得到提高,并具有一定的体外降血糖及抗氧化活性。

To optimize the extraction and purification process of total saponins from chickpea,and evaluate its hypoglycemic and antioxidant activities in vitro.Response surface methodology was used to optimize ultrasound-assisted extraction of total saponins from chickpea;macroporous resin was used for purification;hypoglycemic activity in vitro of total saponins from chickpea was evaluated by inhibiting the activity of alpha-glucosidase;antioxidant activity in vitro was evaluated using total antioxidant capacity,DPPH free radical scavenging capacity,hydroxyl free radical scavenging capacity and reducing capacity.The optimum extraction conditions were as follows:ethanol concentration 68.13%,ultrasound power 106.96 W,ultrasound temperature 49.86℃,and ultrasound time 45.26 min.Under these conditions,the extraction rate was 0.4824%.The purity of total saponins from chickpea increased from 16.42%to 27.81%after purification with D-101 macroporous resin.The inhibitory effects a on alpha-glucosidase(IC50=0.2053 mg/mL),total antioxidant capacity(TEAC=8.408 mmol/g),DPPH free radical scavenging capacity(IC50=0.0667 mg/mL),hydroxyl radical scavenging capacity(IC50=1.836 mg/mL)and reduction capacity of purified total saponins showed a dose-effect relationship,and were stronger than those of non-purified total saponins.By optimizing the extraction and purification process of total saponins from chickpea,the extraction rate and purity of total saponins were improved,and showed considerable hypoglycemic and antioxidant activities.