高密度和高盐度对缢蛏渗透调节和抗氧化水平的影响

Effects of high density and high salinity on osmotic regulation and antioxidant level of Sinonovacula constricta

以缢蛏(Sinonovacula constricta)为实验对象,研究密度(60和120粒/m^(2))和盐度(20和30)对缢蛏渗透调节和抗氧化水平的影响,实验持续72 h,检测了缢蛏血淋巴Na^(+)浓度和K^(+)浓度,肝胰腺组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,以及鳃组织中Na^(+)/K^(+)-ATPase活性、钠氢逆向转运蛋白(Na^(+)/H^(+)antiporter)和膜泡质子泵(V-ATPase)基因表达水平。结果显示:密度和盐度对缢蛏血淋巴Na^(+)浓度、K^(+)浓度无显著交互影响作用,高盐组Na^(+)浓度、K^(+)浓度显著高于对照组;在实验12 h和24 h时,密度和盐度对Na^(+)/K^(+)-ATPase活性的影响具有显著交互作用,并且高盐度组和高密度组Na^(+)/K^(+)-ATPase活性均显著高于对照组;在各时间点,各实验组SOD活性和MDA含量显著高于对照组,且密度和盐度对SOD活性和MDA含量均有显著的交互作用;高盐度组和高密度组Na^(+)/H^(+)antiporter基因和V-ATPase基因的表达均显著高于对照组。由此可见,高密度和高盐度对缢蛏的渗透调节能力和抗氧化水平具有显著交互作用,研究结果对进一步探究海水贝类对密度和盐度变化的响应机制提供了理论参考。

In the present research,the effects of density(60 and 120 individuals/m^(2))and salinity(20 and 30)are examined on osmotic regulation and antioxidant responses of Sinonovacula constricta.The experiment lasted 72 hours.The concentration of Na^(+)and K^(+)in the hemolymph,the activity of SOD and MDA in liver,the activity of Na^(+)/K^(+)-ATPase,and the expression of Na^(+)/H^(+)antiporter and V-ATPase genes in gill were measured.The results showed that density and salinity had no significant interaction on Na^(+)and K^(+)concentration of the hemolymph.Na^(+)and K^(+)concentrations in high-salinity group were significantly higher than those in control group.The effects of density and salinity on Na^(+)/K^(+)-ATPase activity had significant interaction at 12 h and 24 h.Na^(+)/K^(+)-ATPase activity in high-salinity group and high-density group was significantly higher than that in control group.SOD activity and MDA content in all experimental groups were significantly higher than those in control group,and density and salinity had significant interaction on SOD activity and MDA content.The expression of Na^(+)/H^(+)antiporter and V-ATPase genes in high-salinity group and high-density groups were significantly higher than those in control group.Therefore,high density and salinity performed significant interactive effect on osmotic regulation and antioxidant level of S.constricta.The results provided a theoretical reference for further exploring the response mechanism of marine shellfish to density and salinity.