黑豆皮花色苷的超声-微波协同双水相提取工艺

Ultrasonic-microwave Assisted Aqueous Two-phase Extraction of Anthocyanins from Black Soybean Skin

采用超声-微波协同乙醇-硫酸铵双水相的方法对黑豆皮中的花色苷进行提取,探讨双水相体系的硫酸铵用量、乙醇体积分数、pH、液料比、微波功率和提取时间等因素对花色苷提取的影响,采用响应面实验法优化提取工艺.结果表明:以硫酸铵用量21%、40%乙醇溶液组合的乙醇-硫酸铵双水相萃取体系,pH 1.0,液料比60∶1(V/m)、微波提取30 min、微波功率560 W条件下,花色苷得率可达3.055 mg/g.

Anthocyanins is extracted from black soybean skin using ultrasonic-microwave assisted ethamol-ammonium sulfate aqueous two-phase method.The effects of factors such as the amount of ammonium sulfate,alcohol amount,pH value,ratio of solvent and material,microwave power and extraction time on extraction of anthocyanins are investigated.Response surface experiment is used to optimize the extraction process.The results show that under the optimized condition with 21%ammonium sulfate dosage,40%volume fraction of ethanol,60∶1(V/m)ratio of solvent and material of,pH 1.0,560W microwave power and 30min extraction time,the optimal yield of anthocyanins is 3.055mg/g.