小米酒对小鼠血清中相关抗氧化酶活性的影响

Effects of Different Doses of Millet Wine on Activities of Antioxidant Enzymes in Mouse Serum

该实验旨在探究小米酒对小鼠血清中相关抗氧化酶活性的影响。选取SPF级健康KM小鼠适应性饲养一段时间后,将其随机分为6组,分别标记为空白组(0.2 mL生理盐水)、酒基对照组(0.2 mL 16.2%乙醇生理盐水)、阳性对照组(0.2 mL小米原汁)、低剂量组(0.2 mL小米酒)、中剂量组(0.4 mL小米酒)、高剂量组(0.8 mL小米酒)。连续灌胃30 d后,检测小鼠血清中还原型谷胱甘肽(glutathione,GSH)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondialdehyde,MDA)含量变化。结果表明,与酒基组相比,低剂量组小鼠血清中GSH含量和SOD活力显著升高(p<0.05),MDA含量显著降低。说明与同酒精浓度的酒基相比,小米酒可减少氧化应激对肝脏的损伤。

The purpose of this experiment was to investigate the effect of millet wine on the activity of antioxidant enzymes in the serum of mice.Specific pathogen-free and healthy Kunming mice were selected for adaptive breeding prior to random allocation in six groups:blank group(0.2 mL saline),wine-based control group(0.2 mL16.2%ethanol in saline),positive control group(0.2 mL millet juice),low-dose group(0.2 mL millet wine),medium-dose group(0.4 mL millet wine),and high-dose group(0.8 mL millet wine).The treatments were delivered via oral gavage daily for 30 d.Then,the activities of reduced glutathione(GSH)content and superoxide dismutase(SOD)activity were measured and the change in the level of malon dialdehyde(MDA)content was determined.Compared with that in the alcohol-based group,serum GSH content and SOD activity in the lowdose group were significantly increased(p<0.05),and MDA content were significantly reduced.These findings indicate that,compared with alcohol at the same concentration,millet wine can reduce liver damage caused by oxidative stress.