提高人参茎叶皂苷中稀有人参皂苷含量的工艺

The Technology of Increasing the Content of Rare Ginsenosides in Ginseng Stem and Leaf Saponins

以人参茎叶皂苷为底物,用酸水解和微生物转化方法,评价不同方法转化获得稀有人参皂苷含量。通过单因素试验研究酸浓度、温度和底物浓度对稀有皂苷得率的影响;采用正交试验优化工艺条件,确定最佳工艺条件。结果表明,将添加量为0.6%人参茎叶皂苷在0.8%的醋酸溶液中,于110℃下高压反应30 min,稀有皂苷Rg3的含量从1.8%提高到8.82%、Rh2的含量从0.2%提高到1%和Compound K的含量从0.4%提高到6.09%, 3种稀有人参皂苷总量从2%左右提高到15%左右。此条件下,人参茎叶皂苷中高副加值、高药理活性的稀有人参皂苷含量大幅度增加。

The contents of rare ginsenosides were evaluated by acid hydrolysis and microbial transformation. The effects of acid concentration, temperature and substrate concentration on the yield of rare saponins were studied by single factor experiments. The optimum technological conditions were determined by orthogonal test. The results showed that the addition amount of 0.6% of ginseng stems glycosides in 0.8% acetic acid solution, the high pressure reaction at 110 ℃ for 30 min, the content of rare saponin Rg3 increased from 1.8% to 8.82%, Rh2 from 0.2% to 1%, and Compound K from 0.4% to 6.09%, the total amount of 3 rare ginsenosides increased from about 2% to about 15%. Under these conditions, the contents of rare ginsenosides with high side addendum and high pharmacological activity were increased significantly.