干制大鲵皮胶原蛋白提取工艺优化

Optimization of Extraction Process of Collagen from Chinese Giant Salamander’s(Andrias davidianus) Dried Skin

为高效利用大鲵加工副产物大鲵皮,研究干制大鲵皮制备胶原蛋白的工艺条件。以胶原蛋白提取率为评价指标,通过单因素试验法,分别研究胃蛋白酶添加量、酶解pH和酶解时间对大鲵皮胶原蛋白提取率的影响。在单因素试验基础上,通过正交试验法进一步优化大鲵皮制备胶原蛋白工艺,确定最适工艺条件,即胃蛋白酶添加量6000 U/g、酶解pH 2.0、酶解时间6 h,在此工艺条件下,大鲵皮胶原蛋白提取率为86.7%。紫外光谱、红外光谱扫描结果分析表明,用该法提取的干制大鲵皮胶原蛋白保留胶原蛋白较为完整的三股螺旋结构。

In order to utilize the by-products skin of giant salamander,the extraction process of collagen from giant salamander dried skin was studied.The extraction ratio of collagen used as the evaluation index,and the single factor test method was used to study the extraction of collagen from giant salamander dried skin by enzyme addition amount,enzymatic p H and time.On the basis of single factor experiment,the process of the extraction of collagen from giant salamander dried skin was further optimized by orthogonal test,and the optimum technological condition was determined,which was the enzyme addition amount 6000 U/g,pH 2.0 and time 6 h.Under this process condition,the extraction ratio of collagen from giant salamander dried skin was 86.7%.The results of UV and FT-IR showed that the collagen from giant salamander dried skin by this method retained the relatively complete triple helix structure of collagen.