气相色谱法测定茄果类蔬菜中12种有机磷类农药残留

Determination of 12 kinds of organophosphate pesticide residues in solanaceous vegetables by gas chromatography

目的建立气相色谱法同时测定茄果类蔬菜中12种有机磷类农药的残留分析方法。方法样品用乙腈提取、旋蒸后经有机微孔滤膜净化,采用DB-1701毛细管色谱柱程序升温分离,采用火焰光度检测器(flame photometric detector,FPD)测定。结果在本试验条件下,12种有机磷类农药分离效果较好,在0.05~1.0 mg/L范围内呈现良好的线性关系,相关系数r值为0.99935~0.99993,回收率为71.5%~112.4%,RSD值为1.0%~9.4%,检出限为0.01~0.06 mg/kg。结论该方法快速、准确,适合茄果类蔬菜中多种农药残留定量检测要求。

Objective To establish a method for the detection of 12 organophosphate pesticide residues in solanaceous vegetables by gas chromatography.Methods Pesticides in samples were extracted with acetonitrile,then cleaned up using organic millipore filter membrane,and finally separated by a DB-1701 column with temperature programming,and detected quantitatively by hydrogen flame photometric detector.Results 12 organophosphate pesticide residues could be completely separated under this condition,there was a good linear relationship in the range of 0.05-1.00 mg/L,the correlation coefficient r value was 0.99935-0.99993,the recovery of this method was 71.5%-112.4%,the relative standard deviations was 1.0%-9.4%,and the detection limits of this method was 0.01-0.06 mg/kg.Conciusion This method is rapid and accurate,it can be applied to quantitative detection of multi-organophosphate pesticide residues in solanaceous vegetables.