摘要
目的对猴头菇菌丝体多糖热水提取工艺条件进行优化。方法选取影响多糖得率的3个因素:固液比、提取时间和提取温度,在单因素实验的基础上结合L9(34)正交实验,对热水提取法提取猴头菇菌丝体多糖的工艺进行了优化。结果当固液比1:20(m:V,g/mL)、提取时间1.0 h、提取温度70℃,猴头菇菌丝体的多糖得率最高,达(1.76±0.01)%。结论本方法操作简单、快捷、稳定,为猴头菇菌丝体多糖的加工利用和相关研究提供了参考依据。
Objective To optimize the hot water extraction technology for polysaccharides from mycelium of Hericium erinaceus.Methods Three factors including solid-liquid ratio,extraction time and extraction temperature were selected to optimize the extraction process of polysaccharides from Hericium erinaceus mycelium by hot water extraction via L9(34)orthogonal test based on single factor experiments.Results The yield of polysaccharides from Hericium erinaceus mycelium reached the highest(1.76±0.01)%under the following conditions:the solid-liquid ratio was 1:20(m:V,g/mL),the extraction time was 1.0 h,and the extraction temperature was 70℃.Conclusion The optimized method is simple,rapid and stable,which provides a reference for the processing and utilization of Hericium erinaceus mycelium polysaccharides and related research.
作者
王锋
刘晓鹏
张宝翠
朱玉昌
陈莉莉
王应玲
姜宁
WANG Feng;LIU Xiao-Peng;ZHANG Bao-Cui;ZHU Yu-Chang;CHEN Li-Li;WANG Ying-Ling;JIANG Ning(School of Biological Science and Technology,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China;No.2 Geological Brigade of Geological Department of Hubei Province,Enshi 445000,China)
出处
《食品安全质量检测学报》
CAS
2020年第3期771-776,共6页
Journal of Food Safety and Quality
基金
国家自然科学基金项目(81460573)
湖北省技术创新专项(2016AKB058,2017ABA139,2017AKB077)
2019湖北产业教授人才项目
2014年度湖北省本科高校“专业综合改革”试点项目(2014029)
2017湖北民族学院生科院研究生创新计划项目(201709).
关键词
猴头菇
菌丝体
多糖
提取
Hericium erinaceus
mycelium
polysaccharides
extraction
