甲醇超声提取法测定保健食品中维生素A

Determination of vitamin A in health foods by ultrasonic extraction with methanol

目的采用甲醇超声方法提取保健食品中的维生素A。用高效液相色谱法(high performance liquid chromatography,HPLC)法进行含量测定,并与GB/T 5009.82-2016进行比较,同时进行方法学验证。方法样品前处理方法为甲醇超声提取法。采用C18(4.6 mm×250 mm,5μm)色谱柱,甲醇为流动相,检测波长为325 nm,流速为1 mL/min,柱温为30℃,进样量为20μL。结果超声时间为5~15 min提取效率最高,工作曲线在1.00~10.00μg/mL范围内线性关系良好,相关系数为0.9998,相对标准偏差为1.82%(n=6),平均回收率为101.09%,方法检出限为10μg/100 g,定量限为31μg/100 g。结论和国标法相比,本方法操作简单、准确度高,适用于保健食品中维生素A含量的测定。

Objective To extract vitamin A from health foods by ultrasonic extraction with methanol and determine its content by high performance liquid chromatography(HPLC)method and compare with GB/T5009.82-2016.Methods The sample was pretreated with ultrasonic in methanol.The analysis was carried out on a C18 column(4.6 mm×250 mm,5μm)with methanol at the flow rate of 1 mL/min.The detection wavelength was set at 325 mm,column temperature was 30℃and sample size was 20μL.Results The results showed that the extraction rate was highest when the ultrasonic time was 5-15 min,and the linear relationship was good in the range of 1.00-10.00μg/mL(r2=0.9998).The relative standard deviation was 1.82%(n=6),and the average recovery rate was 101.09%.The limit of detection was 10μg/100 g and the limit of quantification was 31μg/100 g.Conclusion Compared to the national standard method,this method is simple and accurate,and is suitable for the determination of vitamin A content in health foods.