QuEChERS-超高效液相色谱-串联质谱法同时测定蔬菜中30种农药残留

Simultaneous detection of 30 pesticide residues in vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

目的应用超高效液相色谱-串联质谱法同时测定蔬菜中30种农药残留。方法蔬菜样品经粉碎后,乙腈超声提取,采用简化的QuEChERS法净化,超高效液相色谱-串联质谱仪检测。结果30种农药在相应浓度范围内线性良好,相关系数r为0.9991~1.0000,在所添加的3个水平浓度下,方法平均回收率为70.6%~102.6%,相对标准偏差为0.3%~9.6%。结论方法操作简便,灵敏度高,分离效果好,回收率高,30种农药的检出下限均低于国家标准,可应用于实际蔬菜样品中多农药残留的同时检测。

Objective To establish a method for simultaneous determination of 30 pesticide residues in vegetables by ultra performance liquid chromatography-tandem mass spectrometry.Methods After crushing,the vegetable samples were extracted by ultrasonic extraction of acetonitrile,purified by simplified QuEChERS method,and detected by ultra performance liquid chromatography-mass spectrometer.Results The 30 pesticides showed good linearity in the corresponding concentration range,and the regression coefficient r was 0.9991-1.0000.Under the 3 added horizontal concentrations,the average recovery was 70.6%-102.6%,and the relative standard deviation was 0.3%-9.6%.Conclusions This method is simple to operate,high sensitivity,good separation effect and high recovery rate.The lower detection limit of 30 kinds of pesticides is lower than the national standard.