气相色谱法测定大蒜精油软胶囊中大蒜素含量的不确定度评定

Uncertainty evaluation for the determination of allicin in garlic essential oil soft capsules by gas chromatography

目的评定气相色谱法测定大蒜精油软胶囊中大蒜素含量的不确定度。方法采用气相色谱法测定大蒜精油软胶囊中大蒜素的含量。根据JJF1059.1-2012《测量不确定度的评定与表示》的有关规定,建立数学模型,分析影响大蒜素含量的不确定度因素,量化各个不确定度分量,合成不确定度。结果气相色谱法测定大蒜精油软胶囊中大蒜素含量的不确定度主要来源于标准溶液配制、样品重复测定和溶液的稀释;测定结果合成不确定度为0.009%,扩展不确定度为0.018%,大蒜素含量测定结果为(1.356±0.018)%,k=2。结论建立的不确定度评定方法适合气相色谱方法测定大蒜精油软胶囊中大蒜素含量不确定度分析,为评价测定方法以及检测结果的准确性提供可靠性依据。

Objective To evaluate the uncertainty of determination of allicin in garlic essential oil soft capsules by gas chromatography.Methods The content of allicin in garlic essential oil soft capsules was determined by gas chromatography.According to JJF1059.1-2012 Measurement uncertainty evaluation and expression,a mathematical model was established,the uncertainty factors affecting allicin content were analyzed,the uncertainty components were quantified and the uncertainty was synthesized.Results The uncertainty of the determination of allicin in garlic essential oil soft capsules by gas chromatography mainly came from the preparation of standard solutions,repeated determination of samples and dilution of solutions.The synthetic uncertainty of the measurement results was 0.009%,the expanded uncertainty was 0.018%,and the determination result of the allicin content was(1.356±0.018)%,k=2.Conclusion The established uncertainty evaluation method is suitable for the uncertainty analysis of the determination of allicin in garlic essential oil soft capsules by gas chromatography,so as to provide reliable basis for evaluating the accuracy of determination methods and test results.