生化培养箱内部的循环风向对霉菌检测结果的影响

Effect of circulating wind direction inside the biochemical incubator on the results of mold detection

目的解决样品在培养过程中出现的污染以及培养基干裂的问题,提高食品检验技术。方法依据GB4789.15-2016《食品安全国家标准食品微生物学检验霉菌和酵母计数》,将生化培养箱进行了改装,通过更换培养箱轴流风机风扇叶,改变培养箱内部循环风向。结果当培养箱内部的循环风方向改变后,改善了样品在培养过程中染菌和培养基干裂的现象,样品在培养过程中染菌几率比改善前降50%,培养基干裂现象完全解决。结论更换培养箱轴流风机风扇叶,改变培养箱内部循环风向,可以有效降低样品的染菌几率,防止培养基干裂对检测结果造成的影响,提高检验结果准确性。

Objective To solve the problems of sample contamination and dry cracking of the culture medium during the cultivation process,and improve food inspection technology.Methods According to GB 4789.15-2016 National food safety standard-Food microbiology inspection-Mold and yeast count,the biochemical incubator was modified by changing the axial flow fan blade of the incubator and the way of the internal circulation wind direction of the incubator.Results When the direction of the circulating air inside the incubator is changed,the phenomenon of bacteria contamination and dry cracking of the medium during the culture process was improved.In the process of culture,the probability of bacterial contamination was 50%lower than that before the improvement,and the phenomenon of culture medium dryness was completely solved.Conclusion Changing the fan blade of the axial flow fan in the incubator and the wind direction of the internal circulation in the incubator can effectively reduce the probability of the sample contaminated with bacteria,prevent the influence of the dry crack of the culture medium on the test results,and improve the accuracy of the test results.