南阳艾内生菌与关键次生代谢产物的相关性探究

Endophytes of Nanyang Mugwort(Artemisia argyi)and Correlation Between Key Communities and Secondary Metabolites

目的通过分析南阳艾不同部位内生菌菌群结构多样性及其与关键次生代谢产物含量之间的关系,探究南阳艾内生菌对其药材品质的影响。方法本研究采用高通量测序技术分析南阳艾不同组织内生菌群落的多样性和组成;利用高效液相色谱技术检测南阳艾不同组织中关键代谢产物的含量;进而通过皮尔逊相关性分析,筛选与南阳艾关键次生代谢产物显著相关的内生菌群落。结果南阳艾内生细菌和真菌多样性和组成具有显著组织差异,根中细菌和真菌多样性指数(Chao和Ace)均显著高于茎和叶。通过LEfSe分析,确定根瘤菌属、链霉菌属、黄单胞菌科、子囊菌纲等为南阳艾生物标记物。相关性分析的结果表明,根瘤菌属、假单胞菌属、黄杆菌属、链霉菌属、曲霉属、油壶菌属等属的丰度与部分苯丙素类和黄酮类代谢物的含量呈现显著正相关(P<0.05)。结论本研究筛选了一些南阳艾次生代谢物显著相关的内生菌群落,为进一步分离南阳艾关键次生代谢产物相关的内生菌株,提升南阳艾的品质提供了科学依据。

Objective To explore the effect of endophytes on Nanyang Mugwort by analyzing the diversity of endophytic flora and its correlation with the content of key secondary metabolites in three compartments of Nanyang Mugwort,and the effect of medicinal material quality.Methods In this study,high-throughput sequencing and high-performance liquid chromatography(HPLC)were carried out to determine the distribution endophytes and key secondary metabolites in three compartments of Nanyang Mugwort respectively.The endophytic communities that were significantly correlated with key secondary metabolites were screened by Pearson correlation analysis.Results We found endophytic diversity and composition showed compartment specificity,and bacterial and fungal alpha diversity values(Chao and Ace)in the root compartment were significantly higher than that of leaf and stem.Linear discriminant analysis efect size(LEfSe)analyses identified some potential microbial biomarkers,such as Rhizobium,Streptomyce,Xanthomonadaceae,and Sordariomycetes.Pearson correlation analysis showed that endophytes(such as Rhizobium,Pseudomonas,Flavobacterium,Streptomyces Aspergillus,and Olpidium)were significantly correlated with contents of some phenylpropanoid and flavonoid metabolites in our study(P<0.05).Conclusion In this study,some endophytic communities with significant correlation between key secondary metabolites of Nanyang Mugwort were identified,which provided valuable information to guide the isolation of endophytic strains related to key secondary metabolites and improve the quality of Nanyang Mugwort.