斑秃动物模型与临床病证特点的吻合度分析

Analysis of the Anastomosis between the Animal Model of Alopecia Areata and the Characteristics of Clinical Disease

本研究基于文献梳理和病证特点赋值法对现有斑秃动物模型进行系统总结,分析其临床吻合度。结果发现,与临床吻合度较高的造模方法有细胞诱导和皮损移植,吻合度高达80%;咪喹莫特乳膏和环磷酰胺药物诱发斑秃动物模型,吻合度为72%。从斑秃疾病的发病机制、致病因素和模型的可复制性、便捷性、实用性等方面考虑,模型动物以C3H/HeJ小鼠和C57BL/6小鼠为首要选择,其他SCID鼠、B6.KM-AA小鼠、SD大鼠、BALB/c小鼠可根据斑秃实验研究内容及周期进行适当选择。分析发现,现有的斑秃动物模型造模方式大多以西医为主,缺少中医致病因素,并且斑秃病证结合模型在实际应用中并不广泛。基于此,建议后续构建斑秃模型时多考虑“病证结合”动物模型,使其更贴合于临床病证特点;可以加入中医肝肾不足、血瘀毛窍、血虚血热不同证型的相关指标及七情内伤等致病因素,增添动物精神状态、饮食饮水量、行为学等表观指标,完善与中西医临床病证特点吻合度高的斑秃动物模型。

In this study,the existing animal models of alopecia areata were systematically summarized based on literature review and disease and syndrome characteristics assignment method,and the clinical anastomosis was analyzed.The results showed that cell induction and skin transplantation had a high anastomosis with the clinic,and the anastomosis was as high as 80%.The anastomosis between imiquimod cream and cyclophosphamide induced alopecia areata was 72%.C3H/HeJ mice and C57BL/6 mice were selected as the first choice in terms of the pathogenesis of alopecia areata disease,pathogenic factors,replicability,convenience and practicability of the model.Other SCID mice,B6.KM-AA mice,SD rats and BALB/c mice can be selected appropriately according to the content and period of the experimental study of alopecia areata.It is found that the existing models of alopecia areata mainly rely on Western medicine,lack of pathogenic factors of traditional Chinese medicine,and the model of combining disease and syndrome of alopecia areata is not widely used in practice.Based on this,it is suggested that the animal model of"combination of disease and syndrome"should be considered in the subsequent construction of alopecia areata model to make it more suitable for clinical characteristics of disease and syndrome.We can add the relevant indicators of different syndrome types of liver and kidney insufficiency,blood stasis and maoqiao,blood deficiency and blood heat and other pathogenic factors,and add the apparent indicators of animal mental state,diet and water intake,behavior,etc.,to improve the animal model of alopecia areata which is highly consistent with the clinical characteristics of traditional Chinese and western medicine.