基于超高效液相色谱-质谱技术的异绿原酸B大鼠体内代谢及排泄研究

Metabolism and Excretion of Isochlorogenic Acid B in Rats by Ultra-High Performance Liquid Chromatography-Mass Spectrometry

目的采用超高效液相色谱-质谱法探究异绿原酸B在大鼠体内的代谢和排泄情况。方法按20 mg·kg-1剂量大鼠灌胃给予异绿原酸B,收集给药前后不同时间点的粪便、尿液、血浆样品,处理后样品经超高效液相色谱-质谱技术进行分析检测。结果根据样品色谱和质谱图分析获得相关化合物的保留时间、质荷比、特征碎片离子再结合相关文献报道,最终22种代谢产物从大鼠粪便、尿液、血浆中被检测出,其中粪便18个、尿液3个、血浆1个。主要代谢途径包括水解、氢化、甲基化、硫酸化等。进一步测定了异绿原酸B及其代谢产物绿原酸在粪便和尿液中的累计排泄量。结论阐明了异绿原酸B在大鼠体内的代谢和排泄情况,为异绿原酸B进一步研究提供实验依据。

Objective The metabolism and excretion of isochlorogenic acid B in rats were investigated by UHPLC-MS.Methods Feces,urine and plasma were individually collected before and at different time points after administration of 20 mg·kg-1.Post-prepared samples were analyzed by UHPLC-MS.Results According to the retention times,m/z,characteristic fragment ions and related literature,a total of 22 metabolites were detected,of which 18 metabolites were present in rat feces,3 metabolites in urine and one metabolite in plasma.The main metabolic pathways contain hydrolysis,hydrogenation,methylation,sulfation and so on.The cumulative excretion of isochlorogenic acid B and its main metabolite chlorogenic acid in feces and urine was further performed.Conclusion The metabolism and excretion of isochlorogenic acid B in rats were explored to provide experimental basis for its further research and development.