基于UPLC-Q-TOF-MS研究加味八珍益母胶囊对卵巢早衰大鼠血清代谢组学的影响

Effect of Jiawei Bazhen Yimu Capsule on Serum Metabolomics in Premature Ovarian Failure Rats Based on UPLC-Q-TOF-MS

目的从代谢组学角度探讨加味八珍益母胶囊对卵巢早衰大鼠的作用机制。方法将雌性SD大鼠随机分为假手术组、模型组及加味八珍益母胶囊低、中、高剂量组,每组10只。采用摘除大鼠双侧卵巢复制卵巢早衰模型,灌胃给药,每日1次,连续21天。通过超高效液相色谱串联飞行时间质谱(Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry,UPLC-Q-TOF-MS)分析各组大鼠血清样本,结合多元统计分析方法,探究加味八珍益母胶囊对卵巢早衰大鼠体内差异代谢物的影响;将MELIN数据库或KEGG数据库鉴定出的差异代谢物导入Metaboanalyst 5.0在线平台进行代谢通路分析。结果共筛选和鉴定出18个潜在差异代谢物,大鼠灌胃加味八珍益母胶囊后绝大部分差异代谢物呈现良好的回调趋势。这18个差异代谢物共富集得到2条代谢通路(Pathway Impact>0.1),分别为甘油磷脂代谢和花生四烯酸代谢通路。结论加味八珍益母胶囊对卵巢早衰的治疗作用可能与其改善血清中差异代谢物水平,恢复大鼠体内正常代谢活动有关。

Objective To explore the mechanism of Jiawei Bazhen Yimu Capsule on premature ovarian failure rats from the perspective of metabolomics.Methods Female SD rats were randomly divided into sham operation group,model group and Jiawei Bazhen Yimu Capsule low,medium and high dose groups,with 10 rats in each group.The model of premature ovarian failure was replicated by removing bilateral ovaries of rats and administered intragastrically once a day for 21 days.The serum samples of rats in each group were analyzed by ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UPLC-Q-TOF-MS).Combined with multivariate statistical analysis,the effects of Jiawei Bazhen Yimu Capsule on differential metabolites in rats with premature ovarian failure were investigated.The differential metabolites identified by MELIN database or KEGG database were imported into Metaboanalyst 5.0 online platform for metabolic pathway analysis.Results A total of 18 potential differential metabolites were screened and identified.Most of the differential metabolites showed a good callback trend after intragastric administration of Jiawei Bazhen Yimu Capsule.These 18 differential metabolites were enriched into 2 metabolic pathways(Pathway Impact>0.1),which were glycerophospholipid metabolism and arachidonic acid metabolism pathways.Conclusion The therapeutic effect of Jiawei Bazhen Yimu Capsule on premature ovarian failure may be related to improving the level of differential metabolites in serum and restoring normal metabolic activities in rats.