艾灸不同腧穴对KOA大鼠不同组织TNF-α、MMP-13表达的影响

Effects of Moxibustion at Different Acupoints on the Expression of TNF-αand MMP-13 in Different Tissues of KOA Rats

目的观察艾灸不同腧穴对膝骨性关节炎(KOA)大鼠血清及关节软骨与滑膜TNF-α、MMP-13表达的影响,探讨艾灸不同腧穴对KOA大鼠的特异性调节作用。方法将大鼠随机分为空白组(Con)、模型组(KOA)、模型+足三里艾灸组(ST36)、模型+阳陵泉艾灸组(GB34)、模型+犊鼻艾灸组(ST35),每组8只。选择关节腔注射MIA溶液的方法制备KOA模型。ST36、GB34、ST35组每日艾灸30 min,7天为1个疗程,共3个疗程。分析各组大鼠的机械刺激痛阈值(MWT),番红固绿染色及OARSI评分,血清、关节软骨与滑膜的TNF-α、MMP-13的表达情况。结果与Con组比,KOA组足底机械痛阈值下降,OARSI评分、血清与关节软骨和滑膜TNF-α以及血清、关节滑膜MMP-13的表达均明显升高(P<0.001,P<0.05)。与KOA组比,3个治疗组足底机械痛阈值上升,OARSI评分、血清TNF-α和MMP-13、软骨TNF-α均降低(P<0.001,P<0.05),软骨MMP-13无明显变化(P>0.05);ST36组滑膜TNF-α和MMP-13低表达(P<0.05);ST35组滑膜MMP-13表达降低(P<0.05);GB34组滑膜TNF-α降低(P<0.01)。与GB34组比,ST36组OARSI评分更低(P<0.05),ST35组血清MMP-13下降显著(P<0.01)。与ST36组比,ST35组的血清TNF-α下降更明显(P<0.01)。结论艾灸不同腧穴治疗KOA表现出腧穴的特异性功效。足三里的作用范围最大,可以从整体和局部都发挥抗炎以及缓解关节损伤的作用;犊鼻侧重从整体抗炎;阳陵泉侧重从局部滑膜抗炎。

Objective To observe the effect of moxibustion at different acupoints on the expression of TNF-αand MMP-13 in serum,articular cartilage and synovium of rats with knee osteoarthritis(KOA),and to explore the specific regulatory effects of moxibustion at different acupoints on KOA rats.Methods Rats were randomly divided into control group(Con),model group(KOA),KOA+Zusanli moxibustion group(ST36),KOA+Yanglingquan moxibustion group(GB34),KOA+Dubi moxibustion group(ST35),8 in each group.The KOA model was prepared by intra-articular injection of MIA solution.ST36,GB34 and ST35 group moxibustion daily for 30 minutes,7d as a course of treatment,a total of 3 courses of treatment.The mechanical withdrawal threshold(MWT),Safranin-fixed green staining and OARSI score,and the expressions of TNF-αand MMP-13 in serum,articular cartilage and synovium were analyzed in each group.Results Compared with the Con group,the MWT in the KOA group was decreased,and the OARSI score,serum and articular cartilage and synovial TNF-α,and serum and synovial MMP-13 expressions were significantly increased(P<0.001,P<0.05).Compared with the KOA group,the three treatment groups had an increased MWT,OARSI score,serum TNF-αand MMP-13,and cartilage TNF-αwere decreased(P<0.001,P<0.05),but cartilage MMP-13 had no significant change(P>0.05);low expression of synovium TNF-αand MMP-13 in ST36 group(P<0.05);decreased expression of synovium MMP-13 in ST35 group(P<0.05);decreased synovium TNF-αin GB34 group(P<0.01).Compared with GB34 group,the OARSI score of ST36 group was lower(P<0.05),and the serum MMP-13 of ST35 group was significantly decreased(P<0.01).Compared with the ST36 group,the serum TNF-αin the ST35 group decreased more significantly(P<0.01).Conclusion Moxibustion of different acupoints for KOA exhibits acupoint-specific effects.Zusanli has the greatest scope of action,exerting both overall and local anti-inflammatory effects and relieving joint damage;Dubi focuses on overall anti-inflammation;Yanglingquan focuses on local synovial anti-inflammation.