紫草炭制前后萘醌类化合物含量变化及止血作用研究

Hemostatic Effects and Content changes of Shikonin Derivatives of Arnebia Euthroma(Royal) Johnst Root after Charcoal Processing

目的采用传统的蒙药炮制方法,对紫草进行炭制,通过紫外可见分光光度法和高效液相色谱法对紫草炭制前后萘醌类化合物进行含量测定,比较原药材和炭制紫草中的含量变化。初步评价紫草炭制品的止血活性。方法UV法测定原药材和炭制紫草的羟基萘醌总色素的含量,HPLC法测定β,β’-二甲基丙烯酰阿卡宁含量。采用毛细管法测定小鼠全血凝血时间和出血时间。结果UV和HPLC的结果表明,炭制紫草的羟基萘醌总色素和β,β’-二甲基丙烯酰阿卡宁的含量均不同程度的下降。云南白药阳性对照组及给药三组均与空白对照组比较具有显著差异(P<0.01),延长全血凝血时间效果明显,且160101C炮制组的凝血时间最短,但是给药组之间均没有太明显的差异。结论本研究可为今后的蒙医药中炭制紫草的质量标准及药理实验提供科学依据。

Objective According to traditional mongolian processing method,Arnebia euchroma(Royal)Johnst root was processed at high temparature for charcoal products.The content changes of skikonin devivates of the raw and its charcoal products were determined by UV-Vis spectrometery and HPLC.Hemostatic effects of Arnebia euchroma(Royal)Johnst root proceeded by different way were primarily investigated.Methods The total content of hydronaphthoquinones in the raw and the processed products was determined by UV-Vis method and the content ofβ,β’-dimethylacrylshikonin was determined by HPLC.The time of total blood clotting and bleeding time in mice were measured by capillary tubemethod Results the contents of hydronaphthoquinones andβ,β’-dimethylacrylshikonin in charcoal products have been decreased at differnent degrees compared with the raw medicine in UV-Vis and HPLC measurments.Positive control group and the three administration groups were compared with the blank control group had significant differences(P<0.01),the effect of prolonging the whole blood clotting time is obvious,and coagulation time of160101 C group is the shortest,but there is no too obvious difference between the drug-administration groups.Conculsion:This study can provide an effective basis for the quality standards and pharmacological experiments of Arnebia euchroma(Royal)Johnst root charcoal products in Mongolian medicine in the future.