基于“味性化味”和网络药理学的藏药三热散治疗慢性胃炎的药性和作用机制研究

Study on the Tharmacological Properties and Action Mechanism of Tibetan Medicine SanRe Powder in Treating Thronic Gastritis Based on “RoNusZhurJes” and Network Pharmacology

藏药三热散是藏医治疗多种寒性疾病的核心方和加减方,也是食疗调节三胃火的常用方,仅在《四部医典》三热散出现频次可达166次,且主要集中在培根、消化不良、慢性胃炎等疾病。本研究基于藏医"味性化味"矢量结构模型和网络药理学等方法,应用Gephi0.9.2和Cytoscape 3.7.0等可视化软件,研究藏药三热散治疗慢性胃炎的药性和作用机制。根据药性矢量分析得出,三热散具有"辛味;苦化味;热锐动轻效"等药性组合突出,具有生热、助消化、促分解、顺行隆等功效,主要对治凉、钝、稳、重等培根特性,主治三胃火衰弱、培根偏盛、胃寒、消化功能障碍病机。根据网络药理学分析,该方治疗慢性胃炎共有2466个预测靶标,主要与B4GALT1、PRKCD、CDK9、HSP90AA1等关键基因靶标相关,其中GO功能分析中与三磷酸腺苷结合、信号转导、蛋白质磷酸化、线粒体等基因有关。KEGG通路分析中与内分泌系统、神经营养因子信号转导、Erb B信号、缝隙连接等通路有关。表明藏药三热散可能通过调节人体免疫功能、信号转导蛋白、能量代谢以及抑制胃肠道细胞癌变等多方面共同干预实现治疗慢性胃炎的作用,这对藏药三热散的临床用药和药性研究具有积极意义,为阐明藏医治疗慢性胃炎潜在机制提供科学依据。

Tibetan medicine SanRe powder is the core prescription and addition and subtraction prescription of Tibetan medicine in treating various cold diseases,and it is also the common prescription of dietary therapy to regulate Three Gastropyretie.Only in the Four Medical Codes,SanRe powder occurs 166 times,and mainly concentrates on Bacon,dyspepsia,chronic gastritis and other diseases.This study used Gephi 0.9.2 and Cytoscape 3.7.0 visualization software to study the pharmacological properties and mechanism of Tibetan medicine SanRe powder in treating chronic gastritis.According to the vector analysis of drug properties,SanRe powder has the prominent combination of"pungent taste;bitter taste;heat sharpening and light effect",and has the functions of heat generation,digestion promotion,decomposition promotion and anterograde.It mainly treats the characteristics of bacon such as cooling,bluntness,stabilization and gravity,and mainly treats the pathogenesis of Three Gastropyretie weakness,excessive bacon,stomach cold and digestive dysfunction.According to the network pharmacological analysis,there are 2466 predictive targets for the treatment of chronic gastritis,mainly related to B4GALT1,PRKCD,CDK9,HSP90AA1 and other key gene targets.GO functional analysis is related to adenosine triphosphate binding,signal transduction,protein phosphorylation,mitochondria and other genes.KEGG pathway analysis is related to endocrine system,neurotrophic factor signal transduction,ErbB signal,gap junction and other pathways.It is suggested that Tibetan medicine SanRe powder may achieve the effect of chronic gastritis by regulating human immune function,signal transduction protein,energy metabolism and inhibiting gastrointestinal cell canceration.It has positive significance for clinical medication and pharmacological research of Tibetan medicine SanRe powder and provides scientific basis for Tibetan medicine to treat chronic gastritis.