大鼠脑内心钠素的免疫组织化学观察

A STUDY OF IMMUNOHISTOCHEMISTRY OF ATRIAL NATRIURETIC FACTOR(ANF)IN THE RAT BRAIN

本文用兔抗心钠素Ⅲ特异血清,用ABC法观察了大鼠脑内心钠素的分布。结果表明心钠素在脑内广泛存在。密集的心钠素免疫反应阳性神经元,主要见于下丘脑视前区、室周核及下丘脑前核等处。密集的心钠素免疫反应阳性纤维布于下丘脑室旁核,终纹床核及终板血管器官等处。心钠素在脑内的分布状态提示心钠素可能具有中枢性调节水电解质平衡及心、血管的功能,此外,亦可能参与下丘脑—垂体系神经内分泌调节的作用。

Using antisera generated in rabbit against atriopeptide Ⅲ(α-ANF 5-28)and immunohistochemical ABC technique,the distribution of atrial natriuretic factor(ANF)was observed in the rat brain.The results showed that a widespread distribution of ANF is present in the brain.The most dense localization of ANF positive perikarya was found in medial preoptic-anteroventral third ventricle and a high concentration of immunoreactive varicose fibers were seen in the paraventricular of hypothalamic nucleus,bed nucleus of the stria terminalis and organum vasculosum laminae terminalis.These findings allow the tentative speculation that ANF could be involved in the central regulation of water and electrolyte balance and that ANF might also be involved in a role of regulation in the neuroendocrine hypothalamo-hypophyseal system.