大鼠迷走神经背核的细胞构筑及其P物质分布的免疫组化研究

THE CYTOARCHITECTURE AND IMMUNOHISTOCHEMICAL STUDY OF THE DISTRIBUTION OF SUBSTANCE P OF THE DORSAL MOTOR NUCLEUS OF VAGUS NERVE IN THE RAT

用图像分析仪测量细胞参数、免疫组化技术结合显微分光光度计测定P物质样免疫反应颗粒的吸光率,对大鼠迷走神经背核的细胞构筑及其P物质的分布情况进行了探讨。1.大鼠迷走神经背核细胞可分为小、中及较大三型,分别占所测细胞总数的50.83%、44.17%及5%。小细胞主要分布于核团吻侧部,中细胞散在于吻、中、尾各部,较大细胞仅位于中间部及尾侧部。各型细胞在核团内、中、外三份的分布无一定规律。2.P物质样免疫反应细胞主要位于大鼠迷走神经背核的尾侧部,大多呈椭圆及圆形,可见1~2个突起,并多数分布于核团的内侧份。3.大鼠迷走神经背核有丰富的P物质样免疫反应纤维,核团吻侧部及中间部纤维呈密集点状,尾侧部为短串珠状。4.核团吻侧部及中间部P物质相对含量明显多于尾侧部。

By using image analyser to investigate parameters of cell and immunohistochemical technique(PAP method)combined with microspectrophotometer to investigate the light absorbance of the substance P-likeimmunoreactive granules,the cytoarchitecture and the distribution of SP of the dorsal motor nucleus of vagus nerve(DMV)in the rat have been studied.The results obtained were as follows:1.Cells of DMV in the rat could be classified into three types,i.e.small-sized,medium-sized and large-sized.They constituted 50.83%,44.17%and 5%of the total number of cells respectively.The small-sized cells were mainly located in the rostral part,the medium-sized were distributed throughout the nucleus,and the large-sized were limited in the middle and caudal parts.No distinct regional differences were observed with respect to the distribution of various types of ceils in medial,intermediate and lateral zones.2.SP-like immunoreactive cells Were distributed mainly in the medial zone of the caudal part of the DMV,most were ovoid and round in shape and one to tyro processes could be observed.3.There were abundant SP-like immunoreactive nerve fibers in the DMV.The fibers in the rostral and middle parts appeared as dense punctate structures,while in the caudal part appeared as short beaded strings.4.The relative value of SP in the rostral and middle parts was significantly higher than that in the caudal part.