家兔环甲肌运动神经的起核及其性质的实验研究

AN EXPERIMENTAL STUDY ON THE ORIGINATING NUCLEI OF THE NERVE TO CRICOTHYROID MUSCLE AND THEIR PROPERTIES IN THE RABBIT

本文用CT-HRP追踪了环甲肌运动神经的起核及其在脑干中的位置,并用AChE组织化学和ENK免疫组化技术观察了它们的性质。家兔环甲肌运动神经元主要起自同侧疑核,约在闩上0.58—2.73mm处,90%以上的标记细胞位于内侧柱。在舌下神经核尾端(闩下1.18—3.18mm)和面后核中也有较多的HRP标记神经元。应用组织化学和免疫组化技术发现:在疑核和舌下神经核中有HRP-AChE双标神经元,在疑核内有HRP-ENK双阳性神经元。这表明家兔起自疑核和舌下神经核的环甲肌运动神经元有些是胆碱能神经元,起自疑核的神经元有的是ENK样反应阳性神经元。本文首次报告了家兔环甲肌运动神经元起自于舌下神经核和面后核以及起核的性质,并对其机能意义进行了讨论。

The originating nuclei of nerve innervating cricothyroid muscle(CTm)and its location in the brain stem were traced with CT-HRP and its property was observed using AChE histochemical and ENK immunohistochemical techniques.In the rabbit CTm motoneurons are located primarily in the rostral third of the ipsilateral ambiguous nucleus,at level 0.58-2.73mm rostral to the obex.More than 90%of the labelling neurons are located in its medial column.There are a number of HRP labelling neurons in the caudal hypoglossal(at levels 1.18-3.18mm caudal to the obex)and retrofacial nuclei.With combined HRP histochemical and immunohistochemical technipues,HRP—AChE double labeled neurons were found in both hypoglossal and ambiguous nuclei,while HRP—ENK double-labeled neurons were seen only in ambiguous nucleus.It suggests that in the rabbit some of the CTm motoneurons arising from the ambiguous and hypoglossal nuclei should be cholinergic,some from the ambiguous nucleus should be ENK-like immunoreaction positive neurons.This paper reported for the first time CTm neurons arising from the hypoglossrl and retrofacial nuclei in the rabbit and the properties of the originating nuclei,Its functional significance was discussed.