补气散寒方对变应性鼻炎小鼠模型的效应机制研究

Study on effect mechanism of Buqi Sanhan Formula on allergic rhinitis mouse model

目的:初步探讨补气散寒方对变应性鼻炎(AR)小鼠模型的作用及可能机制。方法:将BALB/c小鼠随机分为空白对照组、模型组、顺尔宁组及补气散寒方低、中、高浓度组,每组10只。采用卵清蛋白(OVA)致敏建立AR小鼠模型,分别予补气散寒方或孟鲁司特钠灌胃治疗1周,观测记录小鼠症状学积分,观察鼻黏膜组织学改变,血清总免疫球蛋白E(IgE)、OVA特异性IgE以及白介素17A(IL-17A)、白介素33(IL-33)水平。结果:补气散寒方能显著降低变应性鼻炎小鼠鼻部症状,减轻变应性鼻炎所导致的体质量、胸腺质量、胸腺指数的增加,能显著降低血清总IgE、OVA特异性IgE水平,以及降低胸腺中IL-17A、IL-33含量。结论:补气散寒方对AR小鼠疗效确切,其可能通过降低血清总IgE、特异性IgE水平、降低IL-17A水平而发挥治疗变应性鼻炎的效应。

Objective:To explore the effect and possible mechanism of Buqi Sanhan Formula on allergic rhinitis mouse model.Methods:BALB/c mice were randomly divided into blank control group,model group,Singulair group,low,medium and high concentration groups of Buqi Sanhan Formula,10 mice in each group.AR mouse model was established by sensitization with OVA,and was treated with Buqi Sanhan Formula or singulair by gavage for 1 week.The symptomatic scores of the mice were recorded,and histological changes of nasal mucosa,serum levels of total immunoglobulin E(IgE),OVA-specific IgE,interleukin-17A(IL-17A)and interleukin-33(IL-33)were observed.Results:Buqi Sanhan Formula could significantly reduce the nasal symptoms of allergic rhinitis mice,reduce the increase of body weight,thymus weight and thymus index caused by allergic rhinitis,significantly reduce the levels of serum total IgE and ova specific IgE,and reduce the contents of IL-17A and IL-33 in thymus.Conclusion:Buqi Sanhan Formula has definite curative effect on AR mice.It may play a therapeutic effect on allergic rhinitis by reducing the level of serum total IgE,specific IgE and IL-17A.