芪龙祛瘀合剂对缺血性脑卒中的保护作用及其初步机制探究

Study on protective effect and preliminary mechanism of Qilong Quyu Decoction on ischemic stroke

目的:芪龙祛瘀合剂对缺血性脑卒中的保护作用及其初步的机制。方法:建立小鼠大脑中动脉栓塞(MCAO)模型,随机将小鼠分为假手术组、模型组、芪龙祛瘀合剂低剂量组(10 g/kg)、中剂量组(20 g/kg)和高剂量组(30 g/kg);造模结束后灌胃给药,24 h后进行神经功能评分;用TTC染色评估小鼠脑梗死体积;采用免疫组化观察小鼠皮层神经元的数量、小胶质细胞及星形状胶质细胞活化的情况;采用Western Blot检测SOD1、SOD2、CAT和GPX-4的表达;采用ELISA试剂盒检测IL-6、TNF-α和IL-1β的表达。结果:与模型组比较,中剂量和高剂量芪龙祛瘀合剂均能改善小鼠的神经功能损伤及脑梗死体积、改善MCAO后的神经功能缺损(P<0.05)。同时高剂量芪龙祛瘀合剂能够明显减少梗死皮层神经元的损伤,小胶质细胞及星形胶质细胞的激活,升高CAT和GPX-4蛋白水平,减轻脑内ROS的表达,减少IL-6、TNF-α和IL-1β炎症因子的表达(P<0.05)。结论:芪龙祛瘀合剂对小鼠脑缺血再灌损伤有保护作用,其作用机制与其有抗炎及抗氧化作用密切相关。

Objective:To explore the protective effect and preliminary mechanism of Qilong Quyu Decoction on ischemic stroke.Methods:The model of middle cerebral artery occlusion(MCAO)in mouse was established.The mice were randomly divided into sham operation group,model group,Qilong Quyu Decoction low-dose group(10 g/kg),medium-dose group(20 g/kg)and high-dose group(30 g/kg).After modeling,the mice were administered intragastrically,and the neurological function score was evaluated after 24 h.TTC staining was used to evaluate the volume of cerebral infarction in mice.The number of neurons and the activation of microglia and astrocyte in the cortex region were observed by immunohistochemistry.The expressions of SOD1,SOD2,CAT and GPX-4 were detected by Western blot;The epressions of IL-6,TNF-α and IL-1β were detected by ELISA kit.Results:Compared with the model group,medium-dose and highdose Qilong Quyu Decoction group improved the neurological injury,the volume of cerebral infarction and neurological deficit after MCAO in mice(P<0.05).Meanwhile,high-dose Qilong Quyu Decoction significantly reduced the injury of infarcted cortical neurons and the activation of microglia and astrocyte,increased the protein levels of CAT and GPX-4,reduced the level of ROS expression in the brain,and decreased the inflammatory factor expressions of IL-6、TNF-α and IL-1β(P<0.05).Conclusion:Qilong Quyu Decoction has a positive protective effect on cerebral ischemia-reperfusion injury in mice,and its mechanism may be closely related to its anti-inflammatory and antioxidant functions.