基于网络药理学探讨辣椒抗大肠癌的潜在作用机制

Exploring the potential mechanism of capsicum against colorectal cancer based on network pharmacology

目的:基于网络药理学方法分析辣椒抗大肠癌侵袭转移的潜在分子机制。方法:通过TCMSP数据库收集辣椒的主要化学成分及其靶点,通过GeneCards、PharmGkb、OMIM、TTD数据库获取大肠癌相关的疾病靶点,并与活性成分作用靶点取交集即为药物与疾病的共同作用靶点。运用String数据库结合Cytoscape 3.8.2绘制辣椒-大肠癌共同靶点的蛋白相互作用网络图(PPI),筛选核心靶点。用DAVID数据库对关键靶点进行基因本体GO功能富集与KEGG通路分析。结果:辣椒-大肠癌共同靶点260个,核心基因18个。通路分析显示辣椒参与多条大肠癌相关通路的调控,主要涉及PI3K-AKT信号通路、MAPK信号通路、Ras信号通路等经典肿瘤信号通路。结论:辣椒调控多个靶点,同一个靶点参与多个生物学过程和信号通路,辣椒可能通过多靶点、多通路发挥抗大肠癌侵袭转移作用。

Objective:To analyze the potential molecular mechanism of capsicum against colorectal cancer invasion and metastasis based on network pharmacology.Methods:The main chemical components and their targets of capsicum were collected from TCMSP database.The disease targets related to colorectal cancer were obtained from GeneCards,PharmGkb,OMIM and TTD databases,and their intersection with the targets of active ingredients was the common targets of drug and disease.The protein interaction network map(PPI)of capsicum-colorectal cancer common targets was drawn by String database and Cytoscape 3.8.2,and the core targets were screened.GO enrichment analysis and KEGG pathway analysis were performed on key targets using DAVID database.Results:There were 260 common targets and 18 core genes including IGF1R,MAP2K1 and CHEK1.Pathway analysis showed that capsicum was involved in the regulation of several colorectal cancer related pathways,mainly involving the PI3K-AKT signaling pathway,MAPK signaling pathway,Ras signaling pathway and other classic tumor signaling pathways.Conclusion:Capsicum regulates multiple targets,and the same target participates in multiple biological processes and signaling pathways.Capsicum may play an anti-invasion and metastasis role in colorectal cancer through multiple targets and pathways.