酶促反应荧光法测定糖尿病人血浆酮体及初步临床应用

PLASMA KETONE BODIES DETERMINATION BY ENZYMATIG FLUORESGENGE METHOD IN DIABETIGS

本文采用酶荧光法测定正常人及糖尿病人的β-OHBA及ACAC,结果糖尿病组明显高于对照组(P<0.001),糖尿病Ⅰ型明显高于Ⅱ型(P<0.001),糖尿病组的FPG与β-OHBA,ACAC间呈正相关(r分别为0.5214、0.5604),实验说明,本法有助于早期诊治糖尿病酮症及对糖尿病的诊断、分型和病情的监测、治疗。

β-Hydroxybutyrate(β-OHBA)and actoactate(AcAc)were determined by the enzymatic fluorescence method in normal subjects and diabetics.The levels ofβ-OHBA and AcAc were notably increased in diabetic patients(P<0.001),type 1 much more predominant than type 2(P<0.001).In diabetic group,the values of fasting plasma glucose was positively correlated withβ-OHBA and AcAc(r=0.5214 and 0.5604 respectively).Experiment confirmed that the enzymatic fluorescence method is helpful to the early diagnosis of ketosis,and classification,monitoring and treatment of diabetics.