人体皮肤表皮细胞培养和移植

IN VITRO CULTURE OF HUMAN EPIDERMAL CELLS AND THEIR USE IN BURN WOUND COVERAGE

作者采用三种方法,进行了皮肤表皮细胞培养共120例。并初步应用于临床。表皮细胞接种于胶原者,7~10天呈片,形成2~4层细胞,培养结果稳定。但细胞和胶原紧贴,不能单独分离。培养在3T3细胞滋养层者,于2~3周内形成3~8层复层细胞片,生长稳定。传代最多至第六代。由于3T3细胞是异种细胞,故未用于临床。表皮细胞接种于玻璃瓶进行无基底物培养,1~2天就有新细胞增殖,6~10天已形成整张复层细胞片,最多达15层以上,相似于人体皮肤表皮层结构。生长稳定,能进行多次传代,容易从瓶中分脱,适合于移植。作者将培养的细胞膜片移植于削痂创面及供皮区共20例,23例次。移植细胞膜片能加速创面愈合,移植后存活情况满意。

Human epidermal cells from 120 specimens were cultured,using three different methods.Epidermal cells cultured on collagen gel membrane was unsuitable for subcultivation and transplantation because it could not be separated from collagen membrane.Epidermal cells inoculated on 3T3 feeder layer could be subeultured to 6 generations but still unsuitable for transplantation.A new method propagating epidermal cells was to culture epidermal cells in tissue culture flasks without special substrate at 37℃in the presence of 5%CO.Epidermal cells rapidly proliferated and the multilayered sheets of cells were suitable for grafting after 3 weeks.The cultured epithelium was transplanted onto skin donor sites and tangentially excised burn wounds in 20 patients,a 73.5%graft take was obtained.The wounds grafted with cultured cell allografts healed more rapidly.