小牛胸腺细胞抑裂素对人类体外免疫反应的抑制效应

THE IN VITRO SUPPRESSIVE EFFECT OF CALF THYMOCYTE CHALONE ON HUMAN IMMUNE RESPONSIVENESS

小牛胸腺细胞抑裂素对致分裂原(PHA或ConA)、可溶性抗原(破伤风类毒素),同种淋巴细胞体外刺激的人类淋巴细胞反应具有明显的抑制效应,而小牛血清则无此效应。其抑制百分比在浓度为100μg/0.5ml培养液时达90%以上。抑裂素剂量与抑制效应有关,浓度在25μg/0.5ml以下时不明显,剂量增高则加强。

The authors demonstrated that the calf thymocyte chalone suppressed human immune reactions in vitro no matter the lymphocytes were stimulated by mitogens(PHA or Con A),soluble antigen(tetanus toxoid)or allogenic cells.The inhibition percentages(with 100 mcg chalone/0.5 ml)ali exceeded 90%.In contrast,beef serum albumin at same dosage showed very little suppressive effects on those reactions.The suppressive effect of the chalone was not due to the presence of polyamines in the reagent because both the amine oxidase containing calf serum and non-amine oxidase containing pooled human serum used in culture medium had same degree of inhibition effects.The chalone dosago between 25-100 mcg/0.5 ml was found to be directly proportional to the inhibition ability.The prospect of clinical use of chalone as immuno-suppression treatment is discussed.