摘要
定向进化是一个循环过程,在构建多样化基因序列和筛选功能基因变体之间交替进行。该技术目前已被广泛应用于DNA序列、基因功能和蛋白质结构的优化和分析。定向进化包括随机基因文库的生成、基因在合适宿主中的表达和突变文库的筛选。构建基因文库的关键是库容量和突变多样性,而筛选变体的关键是高灵敏度和高通量。文中讨论了定向进化技术的最新进展,这些新技术极大地加速和简化了传统的定向进化过程,为定向进化的发展注入了强劲动力。
Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants.It has been widely used in optimization and analysis of DNA sequence,gene function and protein structure.It includes random gene libraries construction,gene expression in suitable hosts and mutant libraries screening.The key to construct gene library is the storage capacity and mutation diversity,to screen is high sensitivity and high throughput.This review discusses the latest advances in directed evolution.These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.
作者
陈珏
黄佳敏
燕天鹤
彭晓宇
林峻
Jue Chen;Jiamin Huang;Tianhe Yan;Xiaoyu Peng;Jun Lin(Institute of Applied Genomics,Fuzhou University,Fuzhou 350108,Fujian,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,Fujian,China;Fujian Key Laboratory of Marine Enzyme Engineering,Fuzhou University,Fuzhou 350108,Fujian,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2021年第1期163-177,共15页
Chinese Journal of Biotechnology
基金
国家自然科学基金(No.31301537)
福建省科技厅引导性项目(No.2020Y0003)
福建省科技创新联合资金项目(No.2018Y9105)
福建省中青年教师教育科研项目(No.JAT170080)资助
关键词
定向进化
突变文库
随机突变
高通量筛选
半理性设计
directed evolution
mutant library
random mutation
high throughput screening
semi-rational design
