YWHAZ对肾小管上皮细胞增殖的影响

Effect of YWHAZ on the proliferation of renal tubular epithelial cells

目的探索调节蛋白酪氨酸/色氨酸羟化酶激活蛋白ζ(YWHAZ)对正常氧条件以及缺氧复氧处理的肾小管上皮细胞增殖的影响。方法利用人近端肾小管上皮细胞系(HK-2)通过RNAi技术以及质粒转染的方式建立YWHAZ低表达和过表达的细胞模型,观察干预YWHAZ表达后细胞的一般情况,检测细胞增殖活性;而后给予细胞缺氧复氧处理,检测细胞增殖情况的改变。结果成功建立YWHAZ低表达和过表达的细胞模型,低表达YWHAZ后细胞的增殖能力较对照组下降,在缺氧复氧条件下增殖能力下降更为显著,过表达YWHAZ则缓解了缺氧复氧造成的细胞增殖抑制。结论 YWHAZ可能为维持近端肾小管上皮细胞增殖活性的重要因子。

Objective To investigate the effect of regulatory protein tyrosine and tryptophan hydroxylase activator ζ (YWHAZ) on the proliferation of renal tubular epithelial cells under normal oxygen conditions and hypoxic-reoxygenation conditions.Methods Using human proximal renal tubular epithelial cell line(HK-2) through RNAi technology and plasmid transfection to establish a model of low expression and overexpression of YWHAZ, observe the general situation of cells after intervening YWHAZ expression,and detect cell proliferation activity;Then give cells hypoxia-reoxygenation treatment to detect changes in cell proliferation.Results Successfully established YWHAZ low and overexpression cell models.After low expression of YWHAZ, the proliferative capacity of cells decreased compared with the control group, and the proliferative capacity of cells decreased more significantly after hypoxia-reoxygenation treatment;Overexpression of YWHAZ alleviates the inhibition of cell proliferation caused by hypoxia-reoxygenation.Conclusion YWHAZ may be an important factor for maintaining the proliferative activity of proximal tubular epithelial cells.