CXCL10及IFN-γ在上颌窦后鼻孔息肉中的表达及意义

Expression and significance of CXCL10 and IFN-γin antrochoanal polyp

目的探讨炎性细胞、CXC趋化因子配体10(CXC chemokine ligand 10,CXCL10)及干扰素-γ(interferon-γ,IFN-γ)在上颌窦后鼻孔息肉(antrochoanal polyp,ACP)中的表达及意义。方法收集2015年10月至2020年7月于河北北方学院附属第一医院耳鼻咽喉科就诊的58例患者,其中40例ACP患者、18例上颌窦囊肿患者;分别收集手术样本和术前血清;对手术切除标本进行石蜡包埋、HE染色,计数各类炎性细胞的数量与百分比,对ACP患者上皮增生情况进行综合评分。免疫组织化学染色观察CXCL10和IFN-γ在实验组和对照组的表达及差异情况;酶联免疫吸附实验检测两组血清CXCL10的蛋白浓度,并分析蛋白浓度与两组外周血炎细胞间的相关程度。结果HE染色显示ACP组织上皮重构,呈异质性,并有炎细胞浸润,以中性粒细胞、淋巴细胞以及浆细胞为主。血常规炎细胞分析显示中性粒细胞与淋巴细胞呈正相关。免疫组化显示ACP组CXCL10和IFN-γ阳性表达强于对照组。ELISA测定ACP组CXCL10浓度为(32.15±4.60)pg/mL,对照组为(12.11±3.54)pg/mL(P<0.05)。结论CXCL10及IFN-γ在ACP组中的表达含量明显上调,推测与非Ⅱ型炎症(尤其与Th1炎症因子)有关。

Objective To investigate the expression and significance of inflammatory cells,CXC chemokine ligand 10(CXCL10),and interferon-γ(IFN-γ)in the antrochoanal polyp(ACP).Methods A total of 58 samples were collected from the First Affiliated Hospital of Hebei North University between October 2015 and July 2020,including 40 cases of ACP and 18 case of maxillary sinus cysts.Preoperative serum and surgical samples were collected.Paraffin embedding and HE staining were performed on surgical specimens,and the number and percentage of various inflammatory cells were counted.The epithelial hyperplasia of patients with ACP was comprehensively scored.The expression of CXCL10 and IFN-γin ACP and control groups was observed by immunohistochemical staining.The enzyme-linked immunosorbent assay detected the protein concentration of serum CXCL10 in two groups,and the correlation between protein concentration and inflammatory cells in peripheral blood of the two groups was analyzed.Results HE staining revealed obvious epithelial remodeling and heterogeneity with inflammatory cell infiltration,primarily neutrophils,lymphocytes,and plasma cells in group ACP.A positive correlation was found between ACP neutrophils and lymphocytes with the analysis by routine blood examination.Immunohistochemistry showed that CXCL10 and IFN-γin the experimental group were strongly positive compared with those in the control group.The concentration of CXCL10 was(32.15±4.60)pg/mL vs(12.11±3.54)pg/mL in ACP and control groups as determined by ELISA(P<0.05).Conclusion The expression of CXCL10 and IFN-γwere significantly upregulated in the ACP group,which may be related to non-typeⅡinflammation,especially Th1 inflammatory factors.