按揉膏肓穴对肾阳虚型慢性疲劳综合征大鼠的免疫和细胞因子影响研究

Study on the Effect of Pressing and Rubbing Gaofang Points on Immunity and Cytokines for Rats with Kidney-Yang Deficiency Type Chronic Fatigue Syndrome

目的:研究按揉膏肓穴对肾阳虚型慢性疲劳综合征大鼠的免疫和细胞因子影响。方法:按随机数字表将50只大鼠随机分为模型组、膏肓组、肾俞组、药物组和空白组各10只。采用慢性束缚法和强制冷水游泳法制备慢性疲劳模型,予甲基泼尼松龙灌胃,让大鼠呈现“肾阳虚”状态。空白组和模型组每日灌胃喂蒸馏水3ml。膏肓组按揉膏肓穴,肾俞组按揉肾俞穴,药物组予金匮肾气丸配成7.5%溶液的灌胃3ml。以上所有大鼠连续治疗14天。比较治疗后5组生命质量评分、外周T淋巴细胞亚群和血清细胞因子水平。结果:治疗后,与模型组比较,膏肓组、肾俞组和药物组生命质量评分明显改善,且膏肓组改善程度优于肾俞组和药物组,膏肓组、肾俞组和药物T淋巴细胞亚群均明显改善,其中膏肓组在CD3^(+)T淋巴细胞改善程度上优于药物组,在CD8^(+)T淋巴细胞和CD4^(+)/CD8^(+)细胞比值改善程度上优于肾俞组(P均<0.05或0.01);膏肓组、肾俞组和药物组IL-2、IgG、IgA和IgM均明显改善(P均<0.01),这3组在改善程度上无显著差异(P>0.05)。结论:膏肓穴通过更好地调节免疫细胞水平,在治疗肾阳虚型慢性疲乏综合征时,效果较好。

Objective:To study the effect of pressing and rubbing Gaofang points on immunity and cytokines for rats with chronic fatigue syndrome with kidney-Yang deficiency syndrome.Methods:According to the random number table,50 rats were randomly divided into a model group,a Gaofang group,a Shenshu group,a drug group and a blank group with 10 rats each.Chronic restraint method and strong cooling water swimming method were used to prepare chronic fatigue model,and methylprednisolone was administered by gavage to make rats present a“kidney-Yang deficiency”state.The blank group and the model group were fed 3ml of distilled water daily.Pressing and rubbing Gaofang points was given in the Gaofang group,pressing and rubbing Shenshu acupoints was given in the Shenshu group,and 3 ml of a 7.5%Jinkui Shenqi Pills solution was given in the drug group.All the above rats were treated for 14 consecutive days.The quality of life scores,peripheral T lymphocyte subsets and serum cytokine levels of the five groups were compared after treatment.Results:After treatment,compared with the model group,the quality of life scores of the Gaofang group,the Shenshu group and the drug group were significantly improved,and the improvement degree of the Gaofang group was better than that of the Shenshu and the drug group.T lymphocytes Subgroups in the Gaofang group,the Shenshu group and the drug group were all significantly improved,and the Gaofang group was better than the drug group in the improvement of CD_(3)^(+)T lymphocytes,and the improvement in CD_(8)^(+)T lymphocytes and CD_(4)^(+)/CD_(8)^(+)ratio were better than the Shenshu group(P<0.05 or 0.01).The levels of IL-2,IgG,IgA and IgM in the Gaofang group,the Shenshu group and the drug group were significantly improved(all P<0.01),and there was no significant difference in the degree of improvement of these three groups(P>0.05).Conclusion:Gaofang point can better regulate the level of immune cells in the treatment of chronic fatigue syndrome with kidney-yang deficiency syndrome.