原发性肝细胞癌中Id3的表达及临床意义

Expression and clinical significance of Id3 in hepatocellular carcinoma tissues

目的研究分化抑制因子3(Id3)蛋白在原发性肝细胞癌(HCC)中的表达情况,分析Id3与HCC的分化程度、侵袭转移、中医证型、血清甲胎蛋白(AFP)水平等的关系。方法选取2019年11月-2021年2月广州中医药大学第一附属医院确诊的54例HCC病例,采用免疫组化染色EnVision二步法检测Id3蛋白在54例HCC中的表达情况,并以10例癌旁正常肝组织为对照,分析Id3与HCC临床病理特征的相关性。结果Id3主要定位于HCC的细胞核。在54例HCC组织中,Id3呈不同强度的表达,其中标本呈中强阳性表达率为75.9%(41/54),10例正常肝组织Id3中强阳性表达率为10.0%(1/10),前者显著高于后者(P<0.05)。经统计分析,Id3阳性表达强度与年龄及HCC分化程度相关,差异有统计学意义(χ^(2)=6.408、30.351,P均<0.05),即年龄<60岁、分化程度越低的HCC患者,Id3蛋白明显高于年龄≥60岁、分化程度高的HCC患者;但与患者的性别、脉管癌栓、肿瘤大小、Child-Pugh、血清AFP、淋巴结转移、肿瘤数量、有无合并肝硬化及中医证型未见明显相关性,差异无统计学意义(χ^(2)=0.740、2.224、2.427、0.945、1.683、1.521、0.125、1.505、0.289,P均>0.05)。结论Id3蛋白与HCC分化程度相关,其分化程度越低,恶性程度越高,Id3阳性表达越强。Id3表达与年龄相关,年龄越小,Id3表达越强。

Objective To study the expression of inhibitor of differentiation 3(Id3)protein in primary hepatocellular carcinoma(HCC),and analyze the relationship between Id3 and the differentiation degree,invasion and metastasis,traditional Chinese medicine(TCM)syndrome types and serum alpha fetoprotein(AFP)level of HCC.MethodsThe 54HCC cases confirmed in the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from November 2019 to February 2021 were selected.The expression of Id3 protein in 54 cases of HCC was detected by immunohistochemical staining EnVision two-step method,and the correlation between Id3 and clinicopathological features of HCC was analyzed with 10 cases of adjacent normal liver tissues as controls.ResultsId3 was mainly located in the nucleus of HCC.In 54 HCC tissues,Id3 was expressed in different intensities.Among them,the medium strong positive expression rate of specimens was 75.9%(41/54),and the medium strong positive expression rate of Id3 in 10 adjacent normal liver tissues was 10.0%(1/10).The former was significantly higher than the latter(P<0.05).Statistical analysis showed that the positive expression intensity of Id3 was correlated with age and HCC differentiation(χ^(2)=6.408,30.351,P<0.05),that is,the Id3 protein in HCC patients with age<60 years and lower differentiation was significantly higher than that in HCC patients with age≥60 years and high differentiation.However,there were no significant correlation with gender,vascular tumor thrombus,tumor size,Child-Pugh,serum AFP,lymph node metastasis,tumor number,presence or absence of liver cirrhosis and TCM syndrome types(χ^(2)=0.740,2.224,2.427,0.945,1.683,1.521,0.125,1.505,0.289,P>0.05).ConclusionsId3 protein was related to the degree of differentiation of HCC.The lower the degree of differentiation,the higher was the degree of malignancy and the stronger the positive expression of Id3.The expression of Id3 was related to age.The younger the age,the higher was the expression of Id3 level.