一起由诺如病毒GⅠ.5型引起胃肠炎聚集性疫情的分子流行病学分析

Molecular epidemiology of gastroenteritis caused by norovirus type GⅠ.5

目的对2018年深圳市龙华区发生的一起由诺如病毒GⅠ.5型引起的胃肠炎聚集性疫情进行分子流行病分析。方法对诺如病毒GⅠ组核酸阳性的样本进行聚合酶-衣壳区序列测定,同源性和系统进化分析。结果成功获得3份样本的聚合酶-衣壳区序列,样本编号分别为18JB00186、18JB00187和18JB00190,长度552 bp,核苷酸和氨基酸的同源性为100%,与2018年成都分离株MK121731.1同源性最高,相似度(Per.Ident)99.07%。衣壳区的系统发生树构建分析结果显示,它们属于GⅠ.5进化分支,与2013年济南、2014年上海和2018年成都的部分分离株同属于一个进化分支,为GⅠ.5.1分支。聚合酶区的系统发生树构建分析结果显示,它们属于GⅠ.P4进化分支。结论应进一步加强辖区诺如病毒不同型别监测,持续关注不常见型别进化情况。

Objective To investigate the molecular epidemiology of gastroenteritis caused by norovirus GⅠ.5 in Longhua district of Shenzhen city in 2018.Methods The nucleic acid positive samples of norovirus GⅠwere sequenced by polymerase-capsid domain,and analyzed for the homology and phylogenetics.Results The polymerase-capsid region sequences of 3 samples,18 JB00186,18 JB00187 and 18 JB00190,were successfully obtained with 552 bp.The nucleotide and amino acid homology was 100%,which was the same as the 2018 Chengdu isolate MK121731.1.The similarity(Per.Ident)was 99.07%.The phylogenetic tree construction analysis of the capsid region showed that they belonged to the GⅠ.5 evolutionary branch,and belonged to an evolutionary branch of the Jinan,2014 Shanghai and 2018 Chengdu isolates,belonging to the GⅠ.5.1 branch.The phylogenetic tree construction analysis of the polymerase region revealed that they belong to the GⅠ.P4 evolutionary branch.Conclusion Longhua district of Shenzhen city should further strengthen the monitoring of different types of norovirus in its jurisdiction,and continue to pay attention to the evolution of uncommon types.