FGF4对结肠癌细胞迁移的作用

Influence of FGF4 on carcinoma of colon cell migration

目的研究结肠癌组织中成纤维生长因子4(FGF4)对细胞迁移的影响。方法应用重组质粒pCDNA3.1-FGF4和RNA干扰技术分别对低转移结肠癌细胞株T47D和高转移结肠癌细胞株MDA-MB-231中的FGF4进行过表达和干扰并验证,对处理前后的细胞株进行划痕实验。结果高转移结肠癌细胞株MDA-MB-231中FGF4的mRNA水平高于低转移的结肠癌细胞株T47D。T47D-FGF4-OE细胞的划痕距离小于T47D-NC细胞,MDA-MB-231-FGF4-si细胞的划痕距离明显大于MDA-MB-231-NC细胞,差异均有统计学意义(P<0.05),提示MDA-MB-231-FGF4-si细胞的迁移受到明显抑制。结论结肠癌中FGF4的高表达促进了结肠癌细胞株的转移,因此临床上有必要监测FGF4的表达水平,从而有效地评估结肠癌的转移及预后情况。

Objective To study the influence of fibroblast growth factor 4(FGF4)on the migration of colon carcinoma cell.Methods The recombinant plasmid pCDNA3.1-FGF4 and RNA interference were used to express and interfere with FGF4 in low metastatic colon cancer cell line T47D and high metastatic colon cancer cell line MDA-MB-231,respectively.Scratch test was performed on the cell lines before and after treatment.Results The mRNA level of FGF4 in high metastatic colon cancer cell line MDA-MB-231 was higher than that in low metastasis colon cancer cell line T47D.Scratch test was performed on the cell lines before and after treatment,and it was observed that the migration ability of the cell line was significantly improved after successful overexpression of FGF4 in the low metastatic colon cancer cell line T47D,but it was successful in the high metastatic colon cancer cell line MDA-MB-231,the difference is statistically significant(P<0.05).The migration ability of cell lines after interference with FGF4 was significantly reduced.Conclusion FGF4 can highly express in carcinoma of colon and can promote the metastasis of carcinoma of colon cell line.So it is necessary to monitor FGF4 clinical expression level,and effectively evaluate the metastasis and prognosis of carcinoma of colon.