cTfh细胞对乙型肝炎疫苗长期免疫的调控作用

Modulation of Long-term Immunity to Hepatitis B Vaccine by cTfh Cells

目的分析主要辅助B细胞的是滤泡辅助性T(TfhB)细胞对乙型肝炎疫苗长期免疫的调控作用,以提升疫苗免疫应答,遏制乙型肝炎传播。方法经医院伦理学会批准,选择东莞市莞城医院2017年至2019年诊治的乙型肝炎疫苗接种者100例。采用随机数字分组法将其分成对照组和观察组,均50例。对照组患者接受常规乙型肝炎疫苗注射方案。观察组患者使用20μg重组乙型肝炎疫苗(含c Tfh细胞)。比较两组接种者免疫应答情况、乙型肝炎表面抗体(HBsAb)浓度、外周血c Tfh细胞和B细胞、浆细胞和记忆性B细胞含量。结果观察组患者中或强免疫应答率高于对照组(P<0.05),弱或无应答率低于对照组(P<0.05)。观察组在接种后14、28 d HBsAb浓度高于对照组(P<0.05)。观察组接种者在接种14、28 d的cTfh细胞含量高于对照组(P<0.05),B细胞含量低于对照组(P<0.05)。观察组接种者在接种14、28 d的浆细胞含量多于对照组(P<0.05),记忆性B细胞含量低于对照组(P<0.05)。结论cTfh细胞能提升乙型肝炎疫苗长期免疫的调控作用,促使机体免疫应答,增加HBsAb浓度,建议使用。

Objective To investigate the modulation of long-term immunity to hepatitis B vaccine by circulating T-follicular helper(cTfh)cells.Methods One hundred cases of hepatitis B vaccinated patients in Dongguan Guancheng Hospital from 2017 to 2019 were selected with the approval of the hospital ethics committee,and were classified into two groups by random number table method,both with 50 cases.Control group received a conventional hepatitis B vaccination,while observation group were given 20ug of recombinant hepatitis B vaccine containing cTfh cells.Then the immune response,hepatitis B surface antibody(HBsAb)concentration,peripheral blood cTfh cells and B cells,plasma cells and memory B cells were compared between the two groups.Results Patients in observation group had a higher rate of moderate or strong immune response than control group(P<0.05)and a lower rate of weak or non-response than control group(P<0.05).HBsAb concentrations in observation group were higher than those in control group at the 14 d and 28 d of vaccination(P<0.05).The cTfh cell at the 14 d and 28 d of vaccination was higher in observation group than in control group(P<0.05),and B cell content was lower than that in control group(P<0.05).At the 14 d and 28 d of vaccination,observation group had significantly higher levels of plasma cell and lower B cell content(P<0.05).Conclusion cTfh cells can enhance the long-term immunity to hepatitis B vaccine,increase the body's immune response,and elevate the concentration of HBsAb.