多形性胶质母细胞瘤中RAD18的表达及其与放射抵抗的关系

The Expression of RAD18 in Glioblastoma Multiforme and Its Relationship with Radioresistance

目的探究多形性胶质母细胞瘤中RAD18的表达及其与放射抵抗的关系。方法选取2019年8月至2020年8月收治的50例放射治疗后复发及50例放射治疗后未复发的多形胶质母细胞瘤患者作为研究对象,将人脑胶质瘤细胞系(A172)分别转染空白和装有RAD18的质粒载体,应用克隆实验检测经相同剂量射线照射后两组细胞增殖情况,对放射治疗后复发性多形性胶质母细胞瘤样本中RAD18 mRNA的表达情况进行检测,探讨RAD18与多形胶质母细胞瘤放射抵抗的相关性。结果在RAD18蛋白表达上,A172与正常人星形胶质细胞(NHAs)比较,差异无统计学意义(P>0.05);在转染后的RAD18蛋白表达上,转染装有RAD18质粒的A172比转染空白质粒载体高(P<0.05);在γ射线照射后细胞克隆形成数量上,转染装有RAD18质粒的A172比转染空白质粒载体RAD18多(P<0.05);复发性多形胶质母细胞瘤的RAD18蛋白表达比原发性多形胶质母细胞瘤高(P<0.05);RAD18与多形胶质母细胞瘤放射抵抗呈正相关(r=0.608,P<0.05)。结论对多形性胶质母细胞瘤采取放射治疗时会导致DNA损伤,增加RAD18表达,导致DNA的修复能力得到极大提升,进而引起患者放射抵抗,因此临床在放射治疗过程中对RAD18进行抑制,能提升治疗效果。

Objective To explore the expression of RAD18 in glioblastoma multiforme and its relationship with radioresistance.Methods Selected 50 patients with recurrence after radiotherapy and 50 patients with glioblastoma who did not relapse after radiotherapy received from August 2019 to August 2020 as the research objects,and the human glioma cell line(A172).The blank and plasmid vectors containing RAD18 were transfected respectively,and the cell proliferation of the two groups after irradiation with the same dose of radiation was detected by cloning experiment,and the expression of RAD18 mRNA in the samples of recurrent glioblastoma multiforme after radiotherapy was detected.To explore the correlation between RAD18 and radioresistance of glioblastoma multiforme.Results In the expression of RAD18 protein,the difference between A172 and normal human astrocytes(NHAs)was not statistically significant(P>0.05);in terms of the expression of RAD18 protein after transfection,the ratio of A172 transfected with RAD18 plasmid was Transfection blank plasmid vector is high(P<0.05);afterγ-ray irradiation,the number of cell clones formed is more transfected with RAD18 plasmid A172 than transfected blank plasmid vector RAD18(P<0.05);recurrent polymorphic gel the expression of RAD18 protein in glioblastoma was higher than that in primary glioblastoma multiforme(P<0.05);RAD18 was positively correlated with radioresistance of glioblastoma multiforme(r=0.608,P<0.05).Conclusion Radiation therapy for glioblastoma multiforme can cause DNA damage and increase the expression of RAD18,which leads to a greatly improved DNA repair ability,which in turn causes radiation resistance in patients.Therefore,RAD18 is inhibited during the clinical radiotherapy.Can enhance the therapeutic effect.