摘要
为研究大环多胺金属配合物对展青霉素(PAT)水解作用的效果,本文采用1,4,7,10-四氮杂环十二烷(Cyclen)与氯化锰为原料制备出大环多胺锰Cyclen-Mn(Ⅱ)配合物,并通过红外光谱对其结构进行了表征。以Cyclen-Mn(Ⅱ)配合物为模拟酶,利用HPLC法定量研究其在不同温度、pH、时间条件下,对PAT催化水解效果的影响。研究表明:在温度60℃,pH=5.5,水解时间42 h下,Cyclen-Mn(Ⅱ)配合物对PAT的水解率为72%(p<0.05)。该配合物水解PAT的过程服从伪一级动力学模型。经过LC-MS对水解产物进行鉴定,探讨了该配合物对PAT的水解途径,进而剖析其可能的水解机理。Cyclen-Mn(Ⅱ)配合物作为一种人工模拟酶,为PAT的降解提供了一种新思路,在催化及毒理学领域具有潜在应用价值。
The 1,4,7,10-tetraazacyclododecane(cyclen)coordinated with Mn(Ⅱ)(cyclen-Mn(Ⅱ))was synthesized and characterized by FTIR.Batch hydrolysis of patulin by cyclen-Mn(Ⅱ)at different acidities,temperatures and contact times were performed.The concentration of residual patulin was determined by HPLC.At the optimal hydrolysis condition(pH=5.5,60℃for 42 h)(p<0.05),72%of patulin was hydrolyzed by the complex.The rate of hydrolysis process could be well described by pseudo-first-order model.In addition,the hydrolysis product of patulin by cyclen-Mn(Ⅱ)was separated by LC-MS and identified to be C7H8O5.The catalytic mechanism of hydrolysis was proposed.Based on the results,cyclen-Mn(Ⅱ),as an artificial hydrolase mimic,provided a new method for hydrolysis of patulin and may find its applications in catalytic hydrolysis and toxicology areas.
作者
李小品
汪东风
徐莹
LI Xiao-Pin;WANG Dong-Feng;XU Ying(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第S02期53-59,共7页
Periodical of Ocean University of China
基金
国家自然科学基金项目(31871786)资助.
关键词
催化
大环多胺
锰配合物
展青霉素
水解作用
机理
catalysis
tetraazacyclododecane
Mn(Ⅱ)complex
patulin
hydrolysis
mechanism
