摘要
环糊精葡萄糖基转移酶(cyclodextringlycosyltransferase,CGTase)酶法合成环糊精是目前生产环糊精的主要方法。本文介绍了用于生产环糊精葡萄糖基转移酶的几种工程菌株:大肠杆菌、枯草芽孢杆菌以及毕赤酵母,其中大肠杆菌是目前应用最广泛的用于表达CGTase的表达系统。除此之外,本文还总结了高效表达环糊精葡萄糖基转移酶的有效策略:选择合适的表达载体、启动子以及信号肽,以及密码子优化和分子伴侣共表达,以期为在相关CGTase研究领域开展研究提供参考。
The cyclodextrin glycosyltransferases(CGTases)are enzymes that used to synthesize cyclodextrin,and this method of production of cyclodextrin is mostly used at present.We described several engineering strains which were used to produce CGTase including Escherichia coli,Bacillus subtilis and Pichia pastoris,and the expression system of E.coli is one of the most widely used.In addition,we also summarized the effective strategies for high efficient expression of CGTase including appropriate expression vector,promoter and signal peptide,codon optimization,co-expression with molecular chaperone.The purpose of this review is to provide a reference for relevant research of CGTase.
作者
李晓涵
郝建华
郭姣梅
王伟
孙晶晶
刘均忠
LI Xiao-Han;HAO Jian-Hua;GUO Jiao-Mei;WANG Wei;SUN Jing-Jing;LIU Jun-Zhong(Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Drugs and Bioproducts,Yellow Sea Fisheries Research Institute,Qingdao,Shangdong 266071,China;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;Jiangsu Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resource,Lianyungang,Jiangsu 222005,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2020年第2期615-622,共8页
Microbiology China
基金
国家重点研发计划(2018YFC0311106)
中国水产科学研究院基本科研业务费资助(2020TD67)
山东省重点研发计划(2019GHY112030)
青岛市市南区科技发展计划(2018-4-002-ZH).
关键词
环糊精葡萄糖基转移酶
大肠杆菌
枯草芽孢杆菌
酵母
异源表达
策略
Cyclodextrin glycosyltransferase(CGTase)
Escherichia coli
Bacillus subtilis
Yeast
Heterologous expression
Strategy
