利用Ethovision系统对鼠饵适口性筛选的初探

A primary exploration of palatability screening of rat bait with Ethovision system

目的利用Ethovision系统观察鼠类在迷宫内取食饵料的行为特点,从而提高杀鼠饵剂的筛选效率。方法观察分析试鼠在2种迷宫(Phenotyper、T-迷宫)内取食试饵、对照饵的重要参数并进行分析,快速提取出鼠类可能偏好的饵料成分和状态,再结合适口性试验,最后经现场试验验证,获得最优饵料组成。结果在6种试饵(A_(1)~A_(6))中,A_(1)、A_(5)的引诱性结果显著优于其他试饵,A_(1)在Phenotyper逻辑区试鼠行走距离参数(ξ_(d))、停留时间参数(ξ_(t))分别为1.54、1.59,A_(5)在Phenotyper中的ξ_(d)、ξ_(t)值分别为1.87、1.98,A_(5)在T-迷宫中的ξ_(d)、ξ_(t)值分别为1.99、2.27。综合2种迷宫试验结果改进后获得的B_(1)、B_(2)、B_(3)试饵,再次进行T-迷宫实验,B_(1)的ξ_(d)、ξ_(t)值分别为1.94、2.40,引诱性显著高于B_(2)、B_(3)(F=334.895,P<0.001),其第4、12天的摄食系数分别达到1.54、1.62,现场试验盗食率为94.8%。结论Ethovision系统能有效地提高试饵的筛选效率,从而获得适口性更好的饵料。

Objective To introduce the use of the advantage of Ethovision system in observing rat searching food-bait behavior in maze,and so as to raise screening efficiency of rodenticide baits.Methods Observing the behavior of experimental rats feeding on bait-food in 2 kinds of maze test(Phenotyper and T-maze)and analyzing the results comparing with important parameters of baits.The ingredients and condition that rats might prefer were immediately isolated and prepared,and palatability tests were conducted.The optimal bait compositions were obtained based on the test results.Results Among the 6 kinds of baits(A_(1)-A_(6)),A_(1)and A_(5)showed better attractant effect than the others.In Phenotyper logistic area,the walking distance parameter(ξ_(d))and residence time parameter(ξ_(t))of A_(1)were 1.54 and 1.59,while those of A_(5)in Phenotyper were 1.87 and 1.98.ξ_(d)andξ_(t)values of A_(5)in T-maze were 1.99 and 2.27 respectively.Three kinds of test bait,B_(1),B_(2),and B_(3)were designed based on the experimental results in the 2 kinds of mazes.With these baits experiments in T-maze were carried out again.The results were found out thatξ_(d)andξ_(t)values of B_(1)were 1.94 and 2.40 respectively,higher than those of B_(2)and B_(3)(F=334.895,P<0.001).The feeding coefficients of B_(1)on the 4 th and 12th day were 1.54 and 1.62.In field test,the feeding rate on B_(1)was 94.8%.Conclusion Ethovision system can effectively raise the screening efficiency of baits,and help design baits with higher palatability.