精脒对老龄小鼠股骨微结构的影响

Effects of spermidine on bone microstructure of femurs of senile mice

目的探讨精脒(Spermidine)对老龄小鼠股骨微结构的影响。方法选取20只21月龄C57老龄小鼠随机分为对照组(Vehicle,n=10)和实验组(Spermidine,n=10),Spermidine组在饮水中按3 mmol/L的浓度加入Spermidine,干预时间为12周。每周测量各组小鼠的体重;实验结束时,检测血、尿中骨代谢生化指标,micro-CT测量分析股骨形态学指标。结果与Vehicle组相比,Spermidine对老龄小鼠的体重无影响;骨源性碱性磷酸酶(alkaline phosphatase,ALP)水平显著升高,差异有统计学意义(P<0.05);可明显提高骨小梁相对骨体积(trabecular bone volume per tissue volume,Tb.BV/TV)、骨小梁数目(trabecular number,Tb.N)、骨小梁厚度(trabecular thickness,Tb.Th)和骨密度(bone mineral density,BMD),降低骨小梁间隙(trabecular separation,Tb.Sp),差异均有统计学意义(P<0.05)。结论Spermidine能缓解小鼠老龄性骨质疏松。

Objective To investigate the effects of spermidine on bone microstructure of femurs of senile mice.Methods Twenty 21-month-old C57 mice were randomly divided into the vehicle group(n=10)and spermidine group(3 mmol/L spermidine in drinking water,n=10).Body weight was measured weekly during the study.After 12 weeks of intervention,biochemical indexes of bone metabolism in serum and urine were tested and histomorphometry of femurs was measured by micro-CT(microcomputed tomography).Results Compared with the vehicle mice,spermidine had no effect on the weight of senile mice.Spermidine intervention significantly increased serum levels of ALP(P<0.05),elevated the trabecular bone volume per tissue volume(Tb.BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th)and bone mineral density(BMD),and reduced trabecular separation(Tb.Sp).Conclusions Spermidine attenuates age-associated osteoporosis in senile mice.