HPLC法同时测定参龙通络丸中三七皂苷R_(1)和人参皂苷Rf的含量

Simultaneous determination of Notoginsenoside R_(1) and ginsenoside Rf in Shenlong Tongluo pills by HPLC

目的建立一种可以同时测定参龙通络丸中两种成分(三七皂苷R1、人参皂苷Rf)含量的HPLC法。方法采用高效液相色谱法(HPLC),Hypersil Hypersil BDS C18色谱柱(5μm,4 mm×250 mm),乙腈-水为流动相梯度洗脱;流速1.0 ml/min;检测波长203 nm;柱温35℃,检测参龙通络丸中两种成分的含量。结果三七皂苷R1样品量在0.198~9.535μg范围(r=0.9993)、人参皂苷Rf的样品量在0.957~38.392μg范围(r=0.9997)线性关系良好,两种成分的平均回收率分别为99.10%(RSD=1.57%,n=6)和98.76%(RSD=2.13%,n=6)。结论本方法具有结果准确、可靠、重复性好的特点,可为参龙通络丸的质量标准制定提供科学依据。

Objective To establish an HPLC method for simultaneous determination of Notoginsenoside R1 and ginsenoside Rf in Shenlong Tongluo pills.Methods High performance liquid chromatography was used.Hypersil Hypersil BDS C18(5μm,4 mm×250 mm)column was selected with acetonitrile-water as the mobile phase for gradient elution.The flow rate was 1.0 ml/min,detection wavelength was 203 nm and the column temperature was set at 35℃.Results The linear ranges of Notoginsenoside R1 and ginsenoside Rf were 0.198-9.535μg(r=0.9993)and 0.957-38.392μg(r=0.9997)respectively.The average recoveries were 99.10%(RSD=1.57%,n=6)and 98.76%(RSD=2.13%,n=6)respectively.Conclusions This HPLC method was accurate,reliable and reproducible,which can contribute to formulating the quality standard of Shenlong Tongluo pills.