高强度间歇训练对武警战士T淋巴细胞稳态的影响

Effects of high-intensity interval training on T lymphocyte homeostasis in PAP soldiers

目的探讨高强度间歇训练(high-intensity interval training,HIIT)对武警战士T淋巴细胞稳态(Th1/Th2和TH17/Treg)的影响。方法选取23名武警男性战士分别进行急性(1次)和短期(4周、3次/周)HIIT,于1次HIIT运动前、运动后即刻、运动后60 min及4周HIIT前后取静脉血利用流式细胞术测定细胞因子IFN-γ(Th1亚群)、IL-4(Th2亚群)、IL-17A(Th17亚群)、TGF-β(Treg亚群),以及Treg细胞活化标志物CD39、LAP和GARP相对表达量(用阳性细胞率表示)。结果1次HIIT后即刻,Th1细胞IFN-γ表达上调(P<0.05),60 min后,Treg细胞GARP、LAP和CD39表达量升高(P<0.05),IFN-γ恢复(P>0.05);IL-4在各时间点均无显著性变化(P>0.05)。4周HIIT后,Treg细胞LAP表达下调(P<0.05)。未检测到IL-17A和TGF-β表达量。结论1次急性HIIT暂时性破坏Th1/Th2稳态并使Th1/Th2平衡向Th1漂移,运动后恢复期具有免疫抑制作用的Treg细胞被动员,其作用可能参与T细胞稳态的调节与重建。短期(4周)HIIT诱导Treg细胞免疫抑制能力减弱,但T细胞稳态平衡无显著性改变。

Objective To investigate the effects of high-intensity interval training(HIIT)on T lymphocyte homeostasis(Th1/Th2 and Th17/Treg)in PAP soldiers.Methods Twenty-three PAP male soldiers were subjected to acute(a bout)and short-term(4 weeks,3 times/week)HIIT respectively.Venous blood was taken and relative expressions(expressed by positive cell rate)of cytokines of IFN-γ(Th1 subset),IL-4(Th2 subset),IL-17 A(Th17 subset),TGF-β(Treg subset)and Treg activation markers of CD39,LAP and GARP were determined with flow cytometry before,immediately after,60 min after acute HIIT and before and after chronic HIIT.Results Expression levels of IFN-γin Th1 were upregulated(P<0.05)immediately after acute HIIT,while GARP,LAP and CD39 were elevated(P<0.05)and IFN-γrecovered(P<0.05)after 60 min of acute HIIT.IL-4 did not change at any time-point(P>0.05).After 4 weeks of HIIT,LAP in Treg decreased(P<0.05).Expressions of IL-17 A and TGF-βcould not be detected.Conclusions Acute HIIT can transiently perturb Th1/Th2 homeostasis and shift Th1/Th2 balance to Th1,while during recovery,immunosuppressive Treg cells are mobilized to possibly regulate and reconstruct T lymphocyte homeostasis.Short-term(4 weeks)HIIT can reduce immunosuppressive ability of Treg cells without altering T lymphocyte homeostasis.